Detection of bound analyte by magnetic partitioning and masking

Details Detection of bound analyte by magnetic partitioning and masking Detection of bound analyte by magnetic partitioning and masking

1996-07-16

1998-04-21

Wolski, Susan

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

Carrier is inorganic

209214, 210695, 210222, 256307, 256317, 422 57, 422 58, 422 681, 422 8208, 435 75, 435 793, 435 794, 4352872, 436526, 436531, 436534, 436805, 436807, G01N 33553

Patent

active

057417148

ABSTRACT:
A method and apparatus for determining qualitatively or quantitatively the presence of analyte bound to a separation media without doing a bound/free separation. In the method, the bound fraction is collected in an assay region of a body of liquid which includes the free analyte, and the assay is performed by comparing the radiant-energy response in the assay region to the radiant-energy response in a control region of the body of liquid which is free of bound analyte. The apparatus has a chamber which contains the body of liquid, one or more collection elements and a control element and position in the body of liquid parallel to an opaque wall which has a colliminating slit in registry with each element. Each slit enables sensing of the radiant-energy response from the body of liquid between the slit and its associated elements.

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