Chemistry: analytical and immunological testing – Oxygen containing – Hydroxyl containing
Reexamination Certificate
2011-08-02
2011-08-02
Gakh, Yelena G (Department: 1777)
Chemistry: analytical and immunological testing
Oxygen containing
Hydroxyl containing
C436S173000
Reexamination Certificate
active
07989212
ABSTRACT:
A detection method of blood plasma schizadrin B of dissipating blood stasis botanical is disclosed. The method includes: (1) extracting schizadrin B from plasma of mammalian administered dissipating blood stasis botanical by ethyl acetate with the volume ratio 1:4, whirling 3-5 mins, centrifugating at 9600 rpm for 10 mins, drying and enriching the upper layer at 25-30° C., and redissolving with mobile phase; (2) UPLC/MS measuring: UPLC condition: chromatographic column: Acquity UPLC BEH C18, 2.1′100 mm, mobile phase A: water-acetonitrile-formic acid 95:5:0.1 v/v/v, mobile phase B: acetonitrile-formic acid 100:0.1 v/v; MS condition: electric spraying ion source (ESI), detecting with positive ion mode, scanning at the range of m/z 150-1200. The method can be used for pharmacokinetics study of schizadrin B in dissipating blood stasis botanical.
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Ma Yueming
Shi Rong
Zhang Yongyu
Gakh Yelena G
Ren Yunling
Shanghai Sundise
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