Detection of biopolymers and biooligomers with boron hydride lab

Details Detection of biopolymers and biooligomers with boron hydride lab Detection of biopolymers and biooligomers with boron hydride lab

1995-06-02

1997-01-21

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

514 64, C12Q 168, A01N 5508

Patent

active

055958788

ABSTRACT:
A method is disclosed for detecting boron labeled biopolymers, biooligomers, and other species that bind to the biopolymers, and biooligomers with specificity. The biopolymers include RNA, DNA and proteins. The biooligomers include RNA oligomers, DNA oligomers, and peptides. The biopolymers and biooligomers are labeled with a borane, or a boron-hydride moiety. The borane and boron-hydride moiety are employed to reduce a metal ion, e.g. silver, to metal or metal oxide, metal boride or other metal species, or alternatively to reduce a dye, or another organic or inorganic compound. The product of the reduction reaction can be visualized with or without signal augmentation, by one or more means, including the naked eye or a microscope, or can be detected through the use of analytical equipment that can detect conductivity, voltage, density, ultraviolet or visible light, fluorescence, luminescence, phosphorescence, paramagnetism, magnetic susceptibility, or any other detectable physical property. The invention provides methods for selectively detecting biopolymers and biooligomers that contain such a boron-label, thereby permitting their selective identification within an environment where unlabeled like and/or unlike species are present.

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