Detection of biological substances by specific labelling techniq

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 6, 435 28, 436501, 436518, 436827, C12Q 168, C12Q 128, G01N 3353, G01N 33566

Patent

active

046877363

ABSTRACT:
This invention relates to the detection of biological substances and is particularly though not exclusively applicable to labelling techniques employing antibodies or other specific binding agents. Thus the invention consists in a method for the detection of a biological substance of interest (optionally in an environment of biological cells) in which a marker substance is linked specifically, directly or indirectly, to the substance of interest, the marker substance being selected to act as a catalyst in a subsequent process of reduction of silver using a developing solution.

REFERENCES:
patent: 4508820 (1985-04-01), Merril et al.
Putt, F. A., Manual of Histopathological Staining Methods (1972) (Wiley & Sons, Pub.) pp. 278-279.
Jones, P. P., (1977) Journ. of Exp. Med. 146:1261-1279.
Oakley, B. et al., (1980) Anal. Biochem 105:361-63.

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