Detection of binding of charged species using PH- or...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S006120, C424S001210, C514S457000, C549S283000

Reexamination Certificate

active

07056653

ABSTRACT:
The extent of binding of a moiety, such as a biomolecule, to a surface, where the local environment at the surface has a pH or surface potential which is altered upon binding of the moiety, is determined by stably incorporating at the surface a probe which contains a pH- or potential-sensitive fluorophore.

REFERENCES:
patent: 5830912 (1998-11-01), Gee et al.
patent: WO 9711067 (1997-03-01), None
Zuidam et al, “Electrostatic and structural properties of complexes involving plasmid DNA and cationic lipids commonly used for gene delivery”, Biochim. Biophys. Acta (1998) 1368:115-128.
Nicholaas Jan Zuidam and Yechezkel Barenholz, Characterization of DNA-lipd complexes commonly used for gene delivery:, International Journal of Pharmaceutics, vol. 183, pp. 43-46, 1999.
Chatelut, M., et al., “Natural ceramide is unable to escape the lysosome, in contrast to a fluorescent analogue”FEBS Letters 426:102-106 (1998).
Ferrari, M.E., et al., Analytical Methods for the Characterization of Cationic Lipid-Nucleic Acid ComplexesHuman Gene Therapy 9:341-651 (1998).
Fromherz, P., “Lipid Coumarin Dye as a Probe of Interfacial Electrical Potential in Biomembranes”Methods in Enzymology 171:376-387 (1989).
Giudici, M.L., et al., “Uptake and metabolism of fluorescent ceramide analogs by rat oligodendrocytes in culture”FEBS 314(3) :471-476 (1992).
Kraayenhof, R., et al., “Probing Biomembrane Interfacial Potential and pH Profiles with a New Type of Float-like Fluorophores Positioned at Varying Distance from the Membrane Surface”Biochemistry 32:10057-10066 (1993).
Kraayenhof, R., et al., “Monovalent cations differentially affect membrane surface properties and membrane curvature, as revealed by fluorescent probes and dynamic light scattering”Biochimica et Biophysica Acta 1282:293-302 (1996).
Marchesini, S., et al., “A novel fluorescent pH indicator for the acidic range”Biochemistry International 27(3):545-550 (1992).
Pal, R., et al., “Characterization of the Fluorophore 4-Heptadecyl-7-hydroxycoumarin: A Probe for the Head-Group Region of Lipid Bilayers and Biological Membranes”Biochemistry 24:573-581 (1985).
Zelphati, O., et al., “Effect of serum components on the physico-chemical properties of cationic lipid/oligonucleotide complexes and on their interactions with cells”Biochimica et Biophysica Acta 1390:119-133 (1998).
WU, et al., “Hydration and stability of sulfatide-containing phosphatidylethanolamine small unilamellar vesicles,”Biochimica et Biophysica Acta1416:285-294 (1999).
Nichols, “Kinetics of fluorescent-labeled phosphatidylcholine transfer between nonspecific lipid transfer protein and phospholipid vesicles,”Biochemistry27:1889-1896 (1988).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Detection of binding of charged species using PH- or... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Detection of binding of charged species using PH- or..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Detection of binding of charged species using PH- or... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3674266

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.