Detection of beta-galactosidase producing micro-organisms

Details Detection of beta-galactosidase producing micro-organisms Detection of beta-galactosidase producing micro-organisms

1974-09-30

1976-05-18

Naff, David M.

Textiles: fiber preparation

195100, 195102, C12K 104, C12K 110

Patent

active

039575845

ABSTRACT:
A diagnostic test system for detecting .beta.-galactosidase producing micro-organisms is prepared by impregnating one zone of a bibulous material with an aqueous acetone solution of an o-nitrophenyl-.beta.-D-galactopyranoside substrate and evaporating to dryness; and impregnating a contiguous and separate zone of the bibulous material with a solution of a microbiologically inert buffer system which will maintain a pH of from about 6.5 to 8.5 and evaporating to dryness. Preferably, the bibulous material contains 1.5 mg of dry o-nitrophenyl-.beta.-D-galactopyranoside and 0.02 millimoles of a buffer which will maintain a pH of 7.4. Optionally, a waterproof barrier zone and an identifying dye zone may be present. In use, the impregnated bibulous material is inserted into a test tube of a saline suspension of unknown organism and incubated for 4 hours. The development of a yellow coloration indicates that .beta.-galactosidase is produced by the organism being tested. The diagnostic test system of this invention is stable for 1 year at 4.degree.C., at room temperature, and at 37.degree.C.

REFERENCES:
patent: 3359180 (1967-12-01), Evans et al.
patent: 3645853 (1972-02-01), Kronish et al.
patent: 3649461 (1972-03-01), Evans et al.
patent: 3785929 (1974-01-01), Kronish et al.
patent: 3870601 (1975-03-01), Warren et al.
Rosen; R., "Evaluation of the Pathotec `Rapid I-D System` and Two Additional Experimental Reagent-Impregnated Paper Strips," Applied Microbiology, 26(6):pp. 890-893, Dec. 1973.

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