Detection of base pair mismatches using RNAase A

Details Detection of base pair mismatches using RNAase A Detection of base pair mismatches using RNAase A

1985-12-23

1990-08-07

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

4351723, 435803, 436504, 436 63, 436813, 935 2, 935 4, 935 21, 935 23, 935 78, C12Q 168, G01N 3348, C12N 1500

Patent

active

049467735

ABSTRACT:
A method for detecting and localizing single base substitutions in RNA or DNA that involves RNAase A cleavage of single base mismatches in RNA:RNA or RNA:DNA heteroduplexes. A RNA probe complementary to wild type DNA is annealed to the test DNA containing a single base substitution. Many of the possible single base mismatches can be cleaved by RNAase A. The location of the single base substitution can be determined by analyzing the sizes of the RNA cleavage products.

REFERENCES:
patent: 4395486 (1983-07-01), Wilson et al.
patent: 4535058 (1985-08-01), Weinberg et al.
Myers, R. M. et al., "Detection of Single Base Substitutions by Ribonuclease Cleavage . . . ", Science 230, 1242-1246 (13 Dec. 1985).
Winter, E. et al., "A Method to Detect and Characterize Point Mutations . . . .", Proc. Natl. Acad. Sci., U.S.A., 82 (22), 7575-7579 (Nov. 1985).
Langer, P. R. et al., "Enzymatic Synthesis of Biotin-Labeled Polynucleotides . . . ", Proc. Natl. Acad. Sci. 78 (11), 6633-6637 (Nov. 1981).
Orkin et al., Ann. Rev. Genet., vol. 18, p. 131 (1984).
Solomon et al., The Lancet, vol. 1, p. 923 (1979).
Flavell et al., Cell, vol. 15, p. 25 (1978).
Myers et al., Nuc. Acid Res., vol. 13, p. 3111 (1985).
Myers et al., Science, vol. 229, p. 242 (1985).
Wallace et al., Nucl. Acids Res., vol. 6, p. 3543 (1979).
Shenk et al., Proc. Natl. Acad. Sci., U.S.A., vol. 72, p. 989 (1975).
Myers et al., Nuc. Acid. Res., vol. 13, p. 3131 (1985).

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