Detection of bacteria by fluorescent staining in an expanded buf

Details Detection of bacteria by fluorescent staining in an expanded buf Detection of bacteria by fluorescent staining in an expanded buf

1984-01-26

1988-01-05

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 29, 435 34, C12Q 124

Patent

active

047176601

ABSTRACT:
A method for the detection of microorganisms e.g. bacteria in a blood sample comprises staining the microorganisms with a fluorescent dye and observing the fluorescence emission of an expanded buffy coat. The expanded buffy coat is obtained by centrifuging the stained sample in a hematocrit tube containing a float which occupies most of the buffy coat volume. The expanded buffy coat further separates into sub-layers. Intracellular microorganisms congregate in and are detected in the granulocyte sub-layer. The method may be modified to assess phagocytic activity.

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