Detection of antigens via oligonucleotide antibody conjugates

Details Detection of antigens via oligonucleotide antibody conjugates Detection of antigens via oligonucleotide antibody conjugates

1999-06-18

2000-08-29

Brusca, John S.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 6, 536 231, 536 243, 536 245, 536 254, C12Q 168, C07H 2102, C07H 2104, C07H 2100

Patent

active

061106879

ABSTRACT:
The present invention provides a method of detecting antigens, which comprises immobilizing an antigen to a solid support and contacting the solid support with a means for hybridizing a labeled dendrimer to the antibody, through an oligonucleotide complexed thereto. A directly oligonucleotide labeled primary antibody or an oligonucleotide labeled secondary antibody may be employed, and a conventionally labeled dendrimer can subsequently be hybridized to the oligonucleotide through one or more of the outer arms of the dendrimer. The present invention offers the advantage over conventional methods of antigen detection by providing multiple label molecules per antigen, thereby enhancing the observed signal associated with the label.

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