Detection of antibodies against Chlamydia trachomatis pgp3 antig

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435 79, 435 795, 435975, 436518, 436531, G01N 3853

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056291671

ABSTRACT:
A new recombinant form of the plasmid-encoded protein pgp3 from C. trachomatis, serotype D, was purified by ion exchange column chromatography and shown to be suitable for quantitative immunoassy on clinical samples in an ELISA format.

REFERENCES:
patent: 4281061 (1981-07-01), Zuk et al.
Clark et al, "Enzyme linked Immunosorbent Assay (ELISA): Theoretical and Practical Aspects" in Enzyme-Immunoassay published 1987 by CRC Press Inc. pp. 167-179.
Birkelund, S., Dan. Med. Bull, "The Molecular Biology and Diagnostics of Chlamydia trachomatis", 39(4), pp. 304-320, Aug. 1992.
Harlowe et al, "Antibodies: A Laboratory Manual", published 1988 by Cold Spring Harbor Laboratory, Cold Spring (NY), pp. 33-34 and 564.
M. Comanducci, et al., Journal of General Microbiology (1993) 139:1083-1092.
M. Comanducci, et al., Plasmid (1990) 23:149-154.
S. Tabor, et al., Proc. Natl. Acad. Sci. USA, (1985) 262:1074-1078.

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