Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1987-10-22
1989-10-24
Rosen, Sam
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 7, 435 28, 436518, 436536, 436540, C12Q 142, G01N 3353
Patent
active
048761923
ABSTRACT:
A method of detecting the presence of first antibodies against a chorionic gonadotropin-like substance in a first biological sample obtained from an organism other than domestic poultry. The first biological sample is contacted with a chorionic gonadotropin-like substance, preferably isolated from Progenitor cryptocides microorganism, which has been immobilized on a solid phase, under conditions permitting first antibody/chorionic gonadotropin-like substance binding. Unbound sample components are removed from the solid phase, and a plurality of second antibodies, each comprising an immunological conjugate of the first antibody, are contacted with the solid phase, under conditions permitting second antibody/first antibody binding. Unbound second antibodies are removed from the solid phase and the presence of chorionic gonadotropin-like substance/first antibody/second antibody complex, if any, is observed, as a measure of the presence of first antibodies in the first biological sample. Observation can be carried out photometrically by tagging the second antibodies with an enzyme labeling agent, such as alkaline phosphatase or horseradish peroxidase; enzyme degradation of a reagent added to the solid phase can be monitored via a color change reaction. The method can be adapted for use as a screening procedure for conditions associated with elevated levels of antibodies against a chorionic gonadotropin-like substance, such as active and prodromal neoplastic conditions.
REFERENCES:
patent: 4305924 (1981-12-01), Piasio et al.
patent: 4410510 (1983-10-01), Livingstone-Wheeler et al.
patent: 4560649 (1985-12-01), Saxena et al.
patent: 4643970 (1987-02-01), Livingston-Wheeler et al.
Maggio-Enzyme-Immunoassay (1980) CRC, Press, p. 61.
Maruo et al.-Chem. Abst., vol. 92 (1980), p. 90585W.
Koide et al.-Chem. Abst., vol. 94 (1981), p. 170730W.
Bahl, "Human Chorionic Gonadotropin: Purification and Physiochemical Properties", J. Biol. Chem., 244:567-574 (1969).
Livingston et al., "A Specific Type of Organism Cultivated from Malignancy: Bacteriology and Proposed Classification", Ann. N.Y. Acad. Sci., 174:636-654 (1970).
Engvall et al., "Enzyme-Linked Immunosorbent Assay (ELISA) Quantitative Assay of Immunoglobin G", Immunochem., 8:871-874 (1971).
Engvall et al., "Enzyme-Linked Immunosorbent Assay, ELISA: Quantitation of Specific Antibodies by Enzyme-Labeled Anti-Immunoglobulin in Antigen-Coated Tubes", J. Immun., 109:129-135 (1972).
Livingston et al., "Some Cultural, Immunological and Biochemical Properties of Progenitor Cryptocides", Tr. N.Y. Acad. Sci., 36:569-582 (1974).
Voller et al., "Enzyme-Immunoassays for Antibodies in Measles, Cytomegalovirus Infections and After Rubella Vaccination", Br. J. Exp. Path., 57:243-247 (1976).
Lange et al., "Suppression of Antitumor Lymphocyte Mediated Cytotoxicity by Human Chorionic Gonadotropins", J. Urol., 115:95-98 (1976).
Cohen et al., "Bacterial Synthesis of Substance Similar to Human Chorionic Gonadotropin", Proc. Soc. Exp. Biol. Med., 152:408-410 (1976).
Affronti et al., "Production of a Human Gonadotropin-Like Substance by Bacterial Tumor Isolates", Abs. Ann. Meeting Am. Soc. Micro., p. 84, Abs. E23 (1977).
Acevedo et al., "Immunohistochemical Localization of a Choriogonadotropin-Like Protein in Bacteria Isolated from Cancer Patients", Cancer, 41:1217-1229 (1978).
Gosslau et al., "Enzyme-Linked Immunosorbent Microassay for Quantification of Specific Antibodies to Collagen Type I, II, III", J. Immun. Meth. 29:71-77 (1979).
Maruo et al., "Studies on Choriogonadotropin from a Microorganism", The Endocrine Soc., 61st Ann. Meeting, Abst. 951 (1979).
Rennard et al., "Enzyme-Linked Immunoassay (ELISA) for Connective Tissue Components", Anal. Biochem., 104:205-214 (1980).
Chi Tsu T.
Weder Donald E.
Rosen Sam
Seven W. Enterprises, Inc.
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