Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-09-19
1999-03-30
Campbell, Eggerton A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, C12Q 168, C12P 1934
Patent
active
058887407
ABSTRACT:
Disclosed is a method and composition of matter for PCR-based gene dosage analysis. The invention provides internal control DNA sequences that are the same length and same G-C content. The method does not require sized separation of the amplified products. Instead, the method utilizes hybridization and ELISA like colormetric screening. The invention further provides for tightly controlled internal standards for comparing gene dosage by placing one copy of various chromosome markers on one plasmid.
REFERENCES:
patent: 4818708 (1989-04-01), Kerkay
patent: 4940659 (1990-07-01), Warrington et al.
patent: 4983044 (1991-01-01), Schweber
patent: 5213961 (1993-05-01), Bunn et al.
patent: 5252489 (1993-10-01), Macri
patent: 5258907 (1993-11-01), Macri
patent: 5324667 (1994-06-01), Macri
patent: 5324668 (1994-06-01), Macri
patent: 5447841 (1995-09-01), Gray et al.
patent: 5476774 (1995-12-01), Wang et al.
patent: 5506150 (1996-04-01), Canick et al.
patent: 5605843 (1997-02-01), Canick et al.
patent: 5612473 (1997-03-01), Wu et al.
Miller, D., et al., Lancet, Semi-Quantitative Detection of Down's Syndrome with PCR, Sep. 5, 1992, vol. 340 pp. 620-621.
Zheng, Y L, et al., Prenat. Diagn., Analysis of Chromosome 21 Copy Number in Uncultured Amniocytes by Fluorescence in Sita Hybridization Using a Cosmid Contiz., Nov., 1992, 12(11) pp. 931-943.
Peterson, M B, et al., Am J. Hum. Genet., Comparative Study of Microsatellite and Cytogenetic Markers for Detecting the Origin of the Nondisjoined Chromosome 21 in Down Syndrome, Sep., 1992, 51(3) pp. 516-525.
Celi, Francesco, S., Genomics, Determination of Gene Dosage by Quantitative Adaptation of the Polymeoase Chain Reaction (gd-PCR): Rapid Detection of Deletions and Duplications of Gene Sequences, (1994),21 pp. 304-310.
McCormick, Mary Kay, Genomics, Molecular Genetic Approach to the Characterization of the "Down Syndrome Region" of Chromosome 21, (1989),pp. 325-331.
Petersen, Michael B., Am. J. Human. Genet., Use of Short Sequence Repeat DNA Polymorphisms after PCR Amplification to Detect Origin of the Additional Chromosome 21 in Down's Syndrome, (1991), 48 pp. 65-71.
Eggeling, Ferdinand von, Hum. Genet., Rapid Detection of Tresomy 21 by Quantitative PCR, (1993), 91 pp. 567-570.
Pangalos, C., Am. J. Human Genet., Understanding the Mechanism(s) of Mosaic Tresomy 21 by using DNA Polymorphism Analysis, (1994), 54 pp. 473-481.
Pertl, B., Rapid Detection of Trisomies 21 and 18 and Sexing by Quantative Fluorescent Multiplex PCR Hum. Genet. (1996) 98: 55-59.
Lee, Hsien-Hsiung, Human. Genet., Rapid Detection of Trisomy 21 by Homologous Gene Quantitative PCR (HGQ-PCR), (1997), 99 pp.364-367.
Mansfield, E.S., Hum. Mol. Genet., Diagnosis of Down's Syndrome and other Aneuploidies using Quantitative PCR and Small Tandem Repeat Polymorphisms, (1993), 2 (1) pp. 43-50.
Brown et al., Am. J. Med. Genet. 63(2): 373-377. 1996.
Celi et al., Genomics 21:304-310, 1994.
Leu, Science 237:1570. 1987.
Campbell Eggerton A.
Genaco Biomedical Products, Inc.
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