Detection of analytes in a dual-mediator electrochemical...

Electrolysis: processes – compositions used therein – and methods – Electrolytic analysis or testing – Of biological material

Reexamination Certificate

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C204S403140, C204S403110, C435S014000, C435S004000, C435S025000, C205S777500

Reexamination Certificate

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08057659

ABSTRACT:
Determination of an analyte such as glucose in a sample is done making use of a plurality of electron transfer reagents, for example two electron transfer reagents, that work together to transfer electrons between the enzyme and the electrodes. The first electron transfer agent is a mediator that interacts with the enzyme after it has acted on the analyte to regenerate enzyme in its active form. The second electron transfer agent is a shuttle that interacts with the electrodes and optionally the mediator. The oxidation and reduction of the shuttle serves as the major source of current that is measured as an indication of analyte.

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