Detection of analytes having binding sites for at least two bind

Details Detection of analytes having binding sites for at least two bind Detection of analytes having binding sites for at least two bind

1990-06-12

1993-06-29

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 76, 435 78, 436501, 436514, 436517, 436532, 436533, 436534, C12Q 168, C12Q 100, G01N 3353, G01N 33566

Patent

active

052233936

ABSTRACT:
The present invention provides reagents for a detectable label for use in specific binding assays. Specifically, modified .beta.-galactosidase enzyme donors (ED) and acceptors (EA) are utilized. Both ED and EA are modified to form separate ED and EA complexes by coupling each with a linking element and a binding moiety which is specific to a binding site in the analyte. The ED and EA complexes are incapable of forming active enzyme in the absence of the analyte. However, when analyte is present, ED and EA complexes which bind to a common analyte in a sample, active .beta.-galactosidase is formed.

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