Detection of amplified nucleic acid using secondary capture olig

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435 6, 435810, 435 915, 435 911, 536 231, 536 243, 536 2432, 536 2433, 935 16, 935 76, 935 77, 935 78, 935 88, C12Q 168, C07H 1700, C12P 1934

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053875109

ABSTRACT:
A target nucleic acid can be amplified and detected using a water-insoluble capture probe having a short oligonucleotide (15-20 nucleotides) covalently attached to a polymeric particle, and one or more secondary oligonucleotides. The capture probe and secondary oligonucleotides are complementary to the target nucleic acid, although the sequences of complementarity are different. Moreover, each of the secondary oligonucleotides has a length which is equal to or greater than the length of the capture probe oligonucleotide. The secondary oligonucleotides are hybridized to the target nucleic acid either before or during hybridization of the target to the capture probe. The result is improved hybridization efficiency of capture probe and target nucleic acid.

REFERENCES:
patent: 4755458 (1988-07-01), Rabbani et al.
patent: 4965188 (1990-10-01), Mullis et al.
patent: 5079351 (1992-01-01), Sainsky et al.

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