Detection of amplified nucleic acid sequences using bifunctional

Details Detection of amplified nucleic acid sequences using bifunctional Detection of amplified nucleic acid sequences using bifunctional

1996-06-17

1999-11-23

Marschel, Ardin H.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 536 243, 536 253, C12Q 168, C12Q 1934, C07H 2104, C07H 2100

Patent

active

059898135

ABSTRACT:
The invention describes an assay for detecting amplified target nucleic acid sequences with a visual signal. The sensitivity and specificity of the methodology are based on bifunctional target labeling during the amplification step or subsequent hybridization that generates a bifunctional label. The invention may be used, for example, in the screening of amplicon detection for the purpose of more efficiently screening libraries. The invention is also useful to detect nucleic acid sequences indicative of a genetic defect or contagious disease when used with the appropriate primers, as well as detect the existence of nucleic acid amplification.

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