Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2011-03-29
2011-03-29
Horlick, Kenneth R. (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091200
Reexamination Certificate
active
07914986
ABSTRACT:
A method to perform PCR reactions with one set of primers comprising sequence elements that are complementary to the target sequence and comprising sequence elements that server as tagging sequences. By conducting amplification reactions at different temperatures, the presence of contaminations arising from amplification products of previous reactions can be determined, improving reliability of the reaction and reducing the need for control reactions and reproduction of reactions.
REFERENCES:
patent: 6844155 (2005-01-01), Shuber
patent: 99/20798 (1999-04-01), None
Weighardt et al., PCR Methods and Applications 3, 77-80 (1993).
Amplion Limited
Greenberg Laurence A.
Horlick Kenneth R.
Locher Ralph E.
Stemer Werner H.
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