Detection of allele - specific mutagens

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 912, 536 2433, C12Q 168

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active

054966990

ABSTRACT:
Methods are provided for detecting and quantitating gene sequences, such as mutated genes and oncogenes, in biological fluids. The fluid sample (e.g., plasma, serum, urine, etc.) is obtained, deproteinized and the DNA present in the sample is extracted. Following denaturation of the DNA, an amplification procedure, such as PCR or LCR, is conducted to amplify the mutated gene sequence.

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