Detection of a nucleic acid sequence or a change therein

Details Detection of a nucleic acid sequence or a change therein Detection of a nucleic acid sequence or a change therein

1995-04-27

1999-01-05

Sisson, Bradley L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 536 2433, 536 2532, 935 76, 935 77, C12Q 168, C12P 1934, C07H 2100, C12N 1500

Patent

active

058560920

ABSTRACT:
A method for detecting a specific polynucleotide sequence in a material is disclosed. The method includes exposing said material to an oligonucleotide primer having a sequence complementary to part of said specific polynucleotide sequence wherein said primer binds to part of said polynucleotide sequence when present in said material. Primer bound to the polynucleotide sequence is extended wherein any extended primer includes a detectable element and/or a separation element. Any extended primer is then separated into a fraction wherein said fraction does not have detectable element not included in said extended primer. One then determines whether any extended primer is present in said fraction by assaying said fraction for said extended primer wherein the presence of said extended primer is indicative of the presence of the specific polynucleotide sequence in said material and the absence of said extended primer is indicative of the presence of the specific polynucleotide sequences in said material and the absence of said extended primer in said fraction is indicative of the absence of the specific polynucleotide sequence in said material.

REFERENCES:
patent: 4307189 (1981-12-01), Kit
patent: 4656127 (1987-04-01), Mundy
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4851331 (1989-07-01), Vary et al.
patent: 4863849 (1989-09-01), Melamede
Mitchell et al., "Affinity Generation of Single Stranded DNA following the Polymerase Chain eaction: Application to Dideoxy Sequencing," Journal of Cellular Biochemistry, Supplement 13E, Abstracts 18th Annual meeting, WH 214, Apr. 1989.
Smith, "DNA Sequence Analysis by Primed Synthesis" in Methods in Enzymology, vol. 65, pp. 560-581 (1980).
Mitchell, et al. Journal of Cellular Biochemistry, Supplemental 13E, 1989, p. 298.
Sommer et al., "Minimal homology requirements for PCR primers", Nucleic Acids Research, vol. 17, No. 16, 1989, p. 6749.
Ballabio et al., Nature, 343, p. 220 (Jan. 18, 1990).
Grimberg et al., Nuc. Acids Res. 17, p. 8390 (1989).
Signer et al., Nuc. Acids Res. 16, p. 7738 (1988).

Affiliated with

Also associated with

Rating

Detection of a nucleic acid sequence or a change therein does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Detection of a nucleic acid sequence or a change therein, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Detection of a nucleic acid sequence or a change therein will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-860941