Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-05-26
1997-07-01
Sisson, Bradley L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 435 912, 435863, 536 2433, 536 237, 536 2432, 935 8, 935 76, 935 77, 935 78, C12Q 168, C12P 1934, C07H 2104, C12N 1500
Patent
active
056437234
ABSTRACT:
A method is provided for detecting M. tuberculosis or mutants thereof, particularly rifampin-resistant MTB, in a biological sample comprising: isolating DNA from the biological sample; amplifying the isolated DNA under hybridizing conditions with a primer set that targets portions of the gene encoding rpoB; wherein the pimer set comprises at least one primer that hybridizes under hybridizing conditions to at least one signature nucleotide for M. tuberculosis; and isolating and sequencing the amplified DNA to determine the presence or absence of M. tuberculosis or mutants thereof.
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Felmlee Teresa A.
Hunt John J.
Persing David H.
Roberts Glenn D.
Whelan A. Christian
Mayo Foundation for Medical Education and Research
Roche Molecular Systems Inc.
Sisson Bradley L.
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