Chemistry: analytical and immunological testing – Heterocyclic carbon compound – Hetero-o
Patent
1997-08-22
1999-09-28
Soderquist, Arlen
Chemistry: analytical and immunological testing
Heterocyclic carbon compound
Hetero-o
436 93, 436127, 436129, 436131, 436161, 436162, 436169, 422 56, 422 61, G01N 3300, G01N 3090, G01N 3002, G01N 2177
Patent
active
059587857
ABSTRACT:
Chronic or long-term alcohol consumption is detected and diagnosed by determining the levels of an alcohol-specific ethanol glycoconjugate biomarker, identified as ethyl .beta.-glucuronide, in body fluids (e.g. urine) of subjects by calorimetric reaction using qualitative and quantitative assay methods. Ethyl glucuronide has been newly observed and detected as a direct indicator of chronic alcohol consumption, and can be isolated and purified. Economical and reproducible assay methods, such as a chromatographic spot assay, ascending or thin layer chromatography assay, and high pressure liquid chromatography assay provide reliable, objective, and sensitive methods for detecting and monitoring a chronic alcoholic condition. Both the presence of the alcohol-specific ethyl glucuronide and a substantial increase in its levels are diagnostic of chronic alcoholism. Since ethyl glucuronide is produced and appears as a direct response to chronic alcohol intake as determined by the present methods, this carbohydrate is considered to be a unique biomarker for the detection of alcoholism, with virtually no possibility of false positive results.
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Pullarkat Premilia S.
Pullarkat Raju K.
Raguthu Simhachalam
Research Foundation for Mental Hygiene
Soderquist Arlen
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