Detection methods based on HR23 protein binding molecules

Details Detection methods based on HR23 protein binding molecules Detection methods based on HR23 protein binding molecules

2002-11-20

2010-10-26

Dunston, Jennifer (Department: 1636)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S007100, C435S008000, C435S029000

Reexamination Certificate

active

07820374

ABSTRACT:
A method is provided for determining whether an agent is capable of inducing a DNA lesion in a eukaryotic cell, including exposing the eukaryotic cell to the agent and determining whether an HR23 protein-binding molecule accumulates in the cell, where the HR23 protein-binding molecule is preferably xeroderma pigmentosum group C (XPC), 3-methyladenine DNA glycosylase (MAG), CREB, p53, or a functional part, derivative, and/or analogue thereof. Preferably the cell overexpresses HR23A and/or HR23B protein. A rapid and sensitive test is provided with significant advantages over the Ames test. A method is provided for determining whether an agent is capable of inhibiting a cellular process, the process resulting in accumulation of HR23 protein-binding molecule within a cell. A method for determining whether a cell has an impaired DNA repair system is provided. An impaired DNA repair system is indicative for diseases such as xeroderma pigmentosum, cockayne syndrome, and/or trichothiodystrophy.

REFERENCES:
Volker et al. Sequential assembly of the nucleotide excision repair factors in vivo. Molecular Cell. vol. 8, pp. 213-224, Jul. 2001.
Fitch et al. p53 responsive nucleotide excision repair gene products p48 and XPC, but not p53, localize to sites of UV-irradiation-induced DNA damage, in vivo. Carcinogenesis, vol. 24, pp. 843-850, May 2003.
Kroese et al. Genetic tests and their evaluation: can we answer the key questions? Genetics in Medicine, vol. 6, pp. 475-480, 2004.
“analogue.” Merriam-Webster Online Dictionary. 2008. Merriam-Webster Online. May 22, 2008 ,http://www.merriam-webster.com/dictionary/analogue>.
“irradiate.” Merriam-Webster Online Dictionary. 2008. Merriam-Webster Online. May 22, 2008 <http://www.merriam-webster.com/dictionary/irradiate>.
Hoogstraten et al. Versatile DNA damage detection by the global genome nucleotide excision repair protein XPC. Journal of Cell Science, vol. 121, pp. 2850-2859, 2008.
Uchida et al. The carboxy-terminal domain of the XPC protein plays a crucial role in nucleotide excision repair through interactions with transcription factor IIH. DNA Repair, vol. 1, No. 6, pp. 449-461, Jun. 2002.
Masutani et al. Identification and characterization of XPC-binding domain of hHR23B. Molecular and Cellular Biology, vol. 17, No. 12, pp. 6915-6923, Dec. 1997.
Yamaizumi et al., U.v.-induced nuclear accumulation of p53 is evoked through DNA damage of actively transcribed genes independent of the cell cycle, Oncogene, 1994, pp. 2775-2784, vol. 9, No. 10.
Batty et al., Stable Binding of Human XPC Complex to Irradiated DNA Confers Strong Discrimination for Damages Sites, Journal of Molecular Biology, 2000, pp. 275-290, vol. 300, No. 2.
Hey et al., the XPC-HR23B Complex Displays High Affinity and Specificity for Damaged DNA in a True-Equilibrium Fluorescence Assay, Biochemistry, May 28, 2002, pp. 6583-6587, vol. 41, No. 21.
Sugasawa et al., Xeroderma Pigmentosum Group C Protein Complex Is the Initiator of Global Genome Nucleotide Excision Repair, Molecular Cell, Aug. 1998, pp. 223-232, vol. 2, No. 2.
Yokoi et al., The Xeroderma Pigmentosum Group C Protein Complex XPC-HR23B Plays an Important Role in the Recruitment of Transcription Factor IIH to Damaged DNA, Mar. 2000, pp. 9870-9875, vol. 275, No. 13.
Ng et al., Developmental Defects and Male Sterility in Mice Lacking the Ubiquitin-Like DNA Repair Gene mHR23B, Molecular and Cellular Biology, Feb. 2002, pp. 1233-1245, vol. 22, No. 4.
Santagati et al., Different dynamics in nuclear entry of subunits of the repair/transcription factor TFIIH, Nucleic Acids Research, Apr. 1, 2001, pp. 1574-1581, vol. 29, No. 7.
PCT International Search Report, PCT/NL03/00812, Aug. 17, 2004.

Affiliated with

Also associated with

Rating

Detection methods based on HR23 protein binding molecules does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Detection methods based on HR23 protein binding molecules, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Detection methods based on HR23 protein binding molecules will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4225544