Detection method of bio material, fabrication method of chip...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S287100, C435S287200, C435S287900, C435S808000, C436S164000, C436S518000, C436S829000, C436S905000

Reexamination Certificate

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07932046

ABSTRACT:
Provided are a method for detecting biomaterials, a method for fabricating a chip for biomaterial detection and a chip for biomaterial detection. The method for detecting biomaterials is characterized by comprising the steps of: (S1) immobilizing polydiacetylene liposomes onto a substrate; (S2) linking the polydiacetylene liposomes together and layering them on the substrate; (S3) immobilizing a material which forms a complementary binding with a subject biomaterial to be detected onto the polydiacetylene liposomes; (S4) exposing the resulted polydiacetylene liposome to UV light so as to form a chip for biomaterial detection; (S5) applying the subject biomaterial to be detected to the chip for biomaterial detection for reaction; and (S6) measuring a fluorescent signal from the chip for biomaterial detection.

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Jung et al (Small vol. 4, No. 10, pp. 1778-1784, 2008).
Park et al (Biotechnology Journal vol. 3, No. 5, pp. 687-693, 2008).
Park et al (Nanotechnology vol. 19, No. 23, pp. 235103/1-235103/7, 2008).
Park, master's thesis, Chemical Engineering School of Sungkyunkwan University, “Development of label-free sensor system based on arrayed PDS liposme,” Feb. 2008.

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