Detection method for amino acid derivative

Details Detection method for amino acid derivative Detection method for amino acid derivative

1988-08-02

1991-09-24

Hill, Jr., Robert J.

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

Amino acid or sequencing procedure

436 92, 530345, 530408, G01N 2164, G01N 3368

Patent

active

050513699

ABSTRACT:
A method for detecting amino acid derivatives in which 2-anilino-5-thiazolinone derivatives of amino acids are reacted with an amino compound of fluorescein or rhodamine derivative amino compounds to form phenylthiocarbamyl amino acids derivatives. The phenylithiocarbamyl amino acids derivatives are detected with a high level of sensitivity by a fluorescence spectrophotometer.

REFERENCES:
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patent: 4587102 (1986-05-01), Nagatomo et al.
patent: 4861726 (1989-08-01), Stolowitz et al.
patent: 4865994 (1989-09-01), Tsugita et al.
Agr. Biol. Chem., vol. 40, No. 4, 1976, pp. 815-817, K. Muramoto et al., "Analysis of Fluorescein-Thiohydantoin Amino Acids by High Speed Liquid Chromatography".
Analytical Biochemistry, vol. 77, 1977, pp. 569-573, Academic Press, C. L. Zimmerman et al., "Paid Analysis of Amino Acid Phenylthiohydantoins by High-Performance Liquid Chromatography".
Tsugita et al., "Sensitization of Amino Acid Derivatives Obtained from Edman Degradation with Radioactively-Labelled Jodohistamine", J. Biochem., vol. 103, No. 3, pp. 399-401, 1988.

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