Detection method and quantitative analysis method for...

Details Detection method and quantitative analysis method for... Detection method and quantitative analysis method for...

2007-10-02

2007-10-02

Warden, Jill (Department: 1743)

Chemistry: analytical and immunological testing

Hydrogen, per se

Reexamination Certificate

active

10344341

ABSTRACT:
The invention is directed to a method of detecting hydrogen radicals present in water or an aqueous solution, characterized in adding sodium salt of 3,5-dibromo-4-nitrosobenzenesulfonic acid (DBNBS) to a sample for detecting the hydrogen radicals by coloring resulting from absorption characteristics thereof, and further characterized in blowing hydrogen gas into a solution of 1,1-diphenyl-2-piclylhydrazyl (DPPH) having absorption in the vicinity of 517 nm and a solution of sodium salt of 3,5-dibromo-4-nitrosobenzenesulfonic acid (DBNBS) at a constant speed in the presence of platinum black for quantitatively analyzing the concentration of the hydrogen radicals from a calibration curve C of a graph of correlation between absorbance in the vicinity of 450 nm derived from a DBNBS azo compound and the concentration of the formed hydrogen radicals.

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Barr et al., J. Biol. Chem., Jun. 28, 1996, vol. 271, No. 26. pp. 15498-15503.

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