Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1995-11-30
1998-09-22
Redding, David A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
4352886, 422101, 436178, 210767, 210800, 210802, 210348, 2164331, 216434, C12M 300
Patent
active
058112570
DESCRIPTION:
BRIEF SUMMARY
FIELD OF THE INVENTION
This invention relates to apparatus suitable for use in testing liquid samples for the presence of microorganisms.
BACKGROUND OF THE INVENTION
Apparatus for the detection of microorganisms in liquid samples is known, the use of which involves the introduction of the sample, optionally followed by wash liquid, and then growth medium, to encourage the organism to multiply. In some cases, the sample may be an antibiotic which, if residual material remains, can inhibit the growth of organisms thereby giving false negative results. The difficulty encountered with such apparatus is that residual sample may become trapped in corners of the apparatus and is difficult to remove.
SUMMARY OF THE INVENTION
The present invention provides simple apparatus, suitable for manual use, which can obviate the problem described above and provide for both biological detection and optimised sterility testing. The novel assay device comprises a housing having two chambers therewithin, both chambers being partially defined by a filter material and each having a liquid inlet, the device comprising also means for holding the filter material in a first or second position, respectively, such that the chambers are either separate or in communication.
In use of the apparatus, the region of contact between the filter material and the chamber division, where residual sample may collect while the said means is maintained in the first position, can be simply and effectively washed by selecting the second position. A further advantage of the novel apparatus is that is it well adapted to the use of sensitive bioluminescence assays, and by contrast with conventional turbidity observation.
BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 shows a schematic sectional view of a device embodying the present invention.
DESCRIPTION OF THE INVENTION
The invention will now be described by way of example only with reference to FIG. 1, which is a schematic sectional view of a device embodying the present invention.
FIG. 1 shows a generally cylindrical housing 10 having an internal cylindrical wall 11 that effectively divides the housing into respective cylindrical and annular chambers 12, 13. These chambers have respective liquid inlets 14, 15 (although one inlet may be replaced by an internal communication), and are partially defined by a common filter material 16.
FIG. 1 shows also a complementary generally cylindrical part 20 that is engageable with the housing 10, suitably by means of complementary circumferential engagement means 21, e.g., a snap-fit or threaded engagement (if the latter, the housing 10 and part 20 may be held together at all times, in engagement to a greater or lesser degree defining the first and second positions of the filter material 16, as discussed below). The part 20 comprises an internal generally cylindrical wall 22 corresponding to wall 11, and including perforations 23 such that the respective generally cylindrical and annular volumes 24, 25 are in communication. The part 20 also includes a liquid outlet 26.
In use, the housing 10 and part 20 are firmly engaged, such that their respective walls 11 and 22 are substantially in contact, and may compress the filter material 16 therebetween. In this "first" position, the chambers 12 and 13 are separate.
Sample is now introduced through inlet 15 into the chamber 12, and liquid is allowed or caused to drain through the filter material 16 and then out of the device through the outlet 26.
Sample may have collected at the interface of the wall 11 and the filter material 16, and possibly beyond the wall 11. However, the filter material can now be effectively washed by moving or partially disengaging the part 20, so that the filter material 16 is no longer held in close contact with the wall 11, i.e., in the "second position" and introducing a wash material through inlet 14.
A growth medium may now be introduced through the inlet 15 into the chamber 12, to allow the growth of microorganisms retained on the filter material 16 unless that is inhibited by anti
REFERENCES:
patent: 4829005 (1989-05-01), Friedmann et al.
patent: 5358690 (1994-10-01), Guirguis
Celsis International, P.L.C.
Redding David A.
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