Detection and speciation of Campylobacter

Details Detection and speciation of Campylobacter Detection and speciation of Campylobacter

1999-07-30

2000-06-27

Riley, Jezia

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 5, 435 911, 435 912, 536 221, 536 231, 536 243, 536 2431, 536 2433, C12Q 168, C12P 1934, C07H 1900, C07H 2104

Patent

active

060805471

ABSTRACT:
Method for detecting Campylobacter by PCR detection of DNA sequence highly conserved between species lari, coli, jejuni and upsaliensis. Speciation between these four is possible as the PCR product is differentially cleaved by restriction endonucleases.

REFERENCES:
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Birkinhead, D., et al., "PCR for the Detection and Typing of Campylobacters," Lett. Appl. Microbiol. 17:235-237 (1993).
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Giesendorf, B.A.J. et al., "Rapid and Sensitive Detection of Campylobacter spp. In Chicken Products by Using the Polymerase Chain Reaction," Appl. Env. Microbiol. 58:3804-3808 (1992).
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Sigma Chemicla Company, Biochemicals compounds for research and diagnostic reagents p. 1639, 1990.

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