Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1995-08-24
1999-03-30
Naff, David M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 75, 435 79, 435 18, 435180, 530815, 536 221, 536127, 436 22, G01N 3353, G01N 3304, C12N 1108, C07H 106
Patent
active
058887440
ABSTRACT:
Aminoglycosides such as aminoglycoside antibiotics are detected and separated by non-immunoaffinity binding to an immobilized binding protein which is preferably lysozyme or .alpha.-lactalbumin. Aminoglycosides are detected in a biological sample such as milk or a fermentation broth by contacting the sample with the binding protein immobilized on a solid carrier such as particles of carboxylated latex to bind the aminoglycosides to the binding protein, adding a label that binds to the aminoglycosides and measuring the label. In another embodiment, the binding protein containing bound aminoglycosides is separated from the sample, the aminoglycosides are removed from the binding protein, a label is added to the aminoglycosides and the label is measured. Aminoglycosides are removed from a sample by passing the sample through a bioreactor containing the binding protein immobilized on a solid carrier to bind the aminoglycosides to the binding protein and recovering the sample free of aminoglycosides. The immobilized binding protein with bound aminoglycosides may be removed from the bioreactor and the aminoglycosides eluted from the binding protein.
REFERENCES:
patent: 4181636 (1980-01-01), Fischer
patent: 4328311 (1982-05-01), Rowley et al.
patent: 4340736 (1982-07-01), Facella et al.
patent: 4482707 (1984-11-01), Sakakibara et al.
patent: 4693985 (1987-09-01), Degan et al.
patent: 4729951 (1988-03-01), Ferenczy et al.
patent: 4990442 (1991-02-01), Del Campo
patent: 5310565 (1994-05-01), Geyer
Bishop et al., "Detection of Antibiotic/Drug Residues in Milk and Dairy Products", Standard Methods for the Examination of Dairy Products, 16th ed., R.T. Marshall, Ed., American Public Health Assoc., Wash. DC.
Yao et al, "Enzyme-Linked Immunosorbent Assay for the Detection of Fermentation Meltabolites: Aminoglycoside Antibiotics", The Journal of Antibiotics, vol. xxxvii, No. 11, pp. 1462-1468, 1984.
Haagsma et al., "High-Performance Liquid Chromatographic Determination of Spectinomycin in Swine, Calf and Chicken Plasma", J. of Chromatography, vol. 615, pp. 289-295 (1993).
Tsuji et al., "Derivatization of Secondary Amines with 2-Naphtalene-Sulfonyl Chloride . . . ", J. of Chromatography, vol. 333, pp. 365-380 (1985).
Medina et al., "Solid Phase Clean-up and TLC Analysis of Hygromycin B in Bovine Plasma and Swine Serum", in: Residues of Verterinary Drugs in Food, Proceedings of the EuroResidue II Conference, Edited by Haagsma et al., 1993.
Maron et al., "Immunological Studies of Affinity Labelled Hen Egg-White Lysozyme and of the Active Site Region of Related Lysozymes", Biochimica Et Biophysica Acta, vol. 278, pp. 243-249 (1972).
Hancock et al., "Interaction of Aminoglycosides with the Outer Membranes and Purified Lipopolysaccharide and OmpF Porin of Escherichia coli", Ant. Agents and Chem., pp. 1309-1314 (1991).
Medina and Unruh, "Solid-Phase Clean-Up and Thin-Layer Chromatographic Detection of Veterinary Aminoglycosides", J. of Chromatography B, vol. 663, pp. 127-135 (1995).
Shaikh and Allen, "Overview of Physical-Chemical Methods . . . ", J. Assoc. Off. Anal. Chem., vol. 68, No. 5, pp. 1007-1013 (1985).
Medina, "A Competitive Protein Binding Assay for Spectinomycin using Particle Concentration Flourescent Assay", Abstract Index, 108th AOAC International Meeting and Exposition, Portland, Oregon, Sep. 12-15, 1994.
Lai and Sheehan, "Matrix Effects in the Deriviation of Amino Acids . . . ", Bio Techniques, vol. 14, No. 4, pp. 642-649 (1993).
Maitra et al.,"Determination of Aminoglycoside Antibiotics . . . ", Clin. Chem., vol. 25(8), pp. 1361-1367 (1979).
Fernandez-Sousa et al., "On the Inhibition of Hen Egg-White Lisozyme Activity . . . ", Biochem. and Biophy. Res. Com., vol. 75(4), pp. 895-890 (1977).
Scheurer and Moore, "Solid-Phase Extraction of Drugs from Biological Tissues", J. of Anal. Toxi., vol. 15, pp. 264-269 (1992).
Jehl et al., "High-Performance Liquid Chromatography of Antibiotics", J. of Chrom. B, vol. 531, pp. 509-548 (1990).
Sharon and Eshdat, "Affinity Labeling of Lysozyme", Chapter 18, pp. 195-218.
Evangelista and Chen, "Analysis of structural specificty in antibody-antigen reactions . . . ", J. of Chromatography A., vol. 680, pp. 587-591 (1994).
Fagerstam, L., "A Non-Label Technology for Real-Time Biospecific Interaction Analysis", Article submitted for publication in: Techniques in Protein Chemistry II, Vilanfranca, J.J. (ed.), Academic Press, New York (1990).
Medina, J., J. Aric. Food Chem., vol. 34(6), pp. 1046-1049 (1986).
Campbell Naomi F.
Medina Marjorie B.
Fado John
Naff David M.
Poulos Gail E.
Silverstein M. Howard
The United States of America as represented by the Secretary of
LandOfFree
Detection and separation of aminoglycosides by binding to immobi does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Detection and separation of aminoglycosides by binding to immobi, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Detection and separation of aminoglycosides by binding to immobi will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1213528