Detection and quantitation of Neisseria via radioimmunoassay of

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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424 12, 23230B, C12Q 166

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active

042489644

ABSTRACT:
This invention relates to a method for detecting Neisseria bacteria by means of a radioimmunoassay for a bacterial protein. More specifically, this invention employs the method of radioimmunoassay in which a purified enzyme or enzyme related protein from Neisseria cells is labelled and is mixed with the unknown sample which represents a bacterial lysate. A known amount of antibody specific for the enzyme is added to the mixture and forms complexes with the labelled and unlabelled enzyme present. After a suitable reaction period, the labelled enzyme-antibody complex is separated from the mixture, and its radioactivity is determined. The presence and amount of enzyme in the unknown sample is calculated by relating the radioactivity count to a standard curve prepared with known quantities of unlabelled enzyme.

REFERENCES:
patent: 3875011 (1975-04-01), Rubenstein et al.
patent: 3974269 (1976-08-01), Maley
patent: 4029756 (1977-06-01), Gaafar
patent: 4066744 (1978-01-01), Price et al.

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