Detection and quantification of biomolecules using mass...

Details Detection and quantification of biomolecules using mass... Detection and quantification of biomolecules using mass...

2011-03-08

2011-03-08

Riley, Jezia (Department: 1637)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S024300, C536S024330, C536S025300, C536S026600, C435S006120, C435S091100

Reexamination Certificate

active

07902345

ABSTRACT:
The present invention is directed in part to a method for detecting a target nucleic acid using detector oligonucleotides detectable by mass spectrometry. This method takes advantage of the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed oligonucleotide probes from hybridized duplexes and releases labels for detection by mass spectrometry. This process is easily incorporated into a polymerase chain reaction (PCR) amplification assay. The method also includes embodiments directed to quantitative analysis of target nucleic acids.

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