Chemistry: analytical and immunological testing – Biospecific ligand binding assay
Patent
1993-02-03
1994-02-15
Chan, Y. Christina
Chemistry: analytical and immunological testing
Biospecific ligand binding assay
436536, 53038822, 530395, 530413, G01N 33566, G01N 33536, C07K 1528
Patent
active
052866548
ABSTRACT:
An isolated TGF-.beta. supergene family (TSF) receptor polypeptide is provided. This polypeptide preferably is an inhibin/activin receptor polypeptide and has at least 75% sequence identity with the mature human inhibin/activin receptor sequence. Also provided is a method for purifying TGF-.beta. supergene family members such as inhibin or activin using the polypeptide, and a method for screening for compounds with TGF-.beta. supergene family member activity by contacting the compound with the polypeptide and detecting if binding has occurred and the compound is active.
REFERENCES:
Georgi et al., Cell, 1:635-645 (1990), "dat-1, a C. Elegans Gene Controlling Dauer Larva Development, Encodes a Novel Receptor Protein Kinase".
Kondo et al., BBRC, 181(2):684-690 (1991), "Activin Receptor mRNA is Expressed Early in Xenopus Embryogenesis and the Level of the Expression Affects the Body Axis Formation".
Bilezikjian et al., Mol. Endocr., 5:1389-1395 (1991), "Activin-A Inhibits Proopiomelanocortin Messenger RNA Accumulation and Adrenocorticotropin Secretion of AtT20 Cells".
Vale et al. in Peptide Growth Factors and Their Receptors II, Sporon et al., eds., Springer-Verlag, 1990, pp. 211-248, "The Inhibin/Activin Family of Hormones and Growth Factors".
Nakamura et al., J. Biol. Chem., 266(29):19432-19437 (1991), "Follistatin, an Activin-binding Protein, Associates with Heparan Sulfate Chains of Proteoglycans on Follicular Granulosa Cells".
Legerski et al., BBRC, 183(2):672-679 (1992), "Molecular Cloning and Characterization of a Novel Rat Activin Receptor".
Woodruff et al., Endocrin., 130(2):871-881 (1992), "Stage-Specific Binding of Inhibin and Activin to Subpopulations of Rat Germ Cells".
Campen and Vale, BBRC, 157(2):844-849 (1988), "Characterization of Activin A Binding Sites on the Human Leukemia Cell Line K562".
Sugino et al., J. Biol. Chem., 263(30):15249-15252 (1988), "Identification of a Specific Receptor for Erythroid Differentiation Factor on Follicular Granulosa Cell".
Kojima and Ogata, BBRC, 159(3):1107-1113 (1989), "Dual Effect of Activin A On Cell Growth in BALB/C 3T3 Cells".
LaPolt et al., Mol. Endocrin., 3:1666-1673 (1989), "Activin Stimulation of Inhibin Secretion and Messenger RNA Levels in Cultured Granulosa Cells".
Centrella, et al., Mol. & Cell. Biol., 11(1):250-258 (1991), "Activin-A Binding and Biochemical Effects in Osteoblast-Enriched Cultures from Fetal-Rat Parietal Bone".
Hino et al., J. Biol. Chem., 264(17):10309-10314 (1989), "Characterization of Cellular Receptors for Erythroid Differentiation Factor on Murine Erythroleukemia Cells".
Kondo et al., BBRC, 161(3):1267-1272 (1989), "Identification of the Two Types of Specific Receptor for Activin/EDF Expressed on Friend Leukemia and Ambryonal Carcinoma Cells".
Nakamura et al., Science, 247:836-838 (1990), "Activin-Binding Protein from Rat Ovary is Follistatin".
Ignotz and Massague, Cell, 51:189-197 (1987), "Cell Adhesion Protein Receptors as Targets for Transforming Growth Factor-.beta.Action".
Massague et al., Annals NY Acad. Sci., 593:59-72 (1990), "TGF-.beta. Receptors and TGF-.beta. Receptors Binding Proteoglycans: Recent Progress in Identifying Their Functional Properties".
Laiho et al., J. Biol. Chem., 265(30):18518-18524 (1990), "Concomitant Loss of Transforming Growth Factor (TGF)-.beta. Receptor Types I and II in TGF-.beta.-Resistant Cell Mutants Implicates Both Receptor Types in Signal Transduction".
Matzuk and Bradley, Biochimica et Biophysica Acta, 1130:105-108 (1992), "Cloning of the Human Activin Receptor cDNA Reveals High Evolutionary Conservation".
de Winter et al., Mol. and Cell. Endocrin., 83:R1-R8 (1992), "Activin Receptor mRNA Expression in Rat Testicular Cell Types".
Mathews et al., Science, 255:1702-1705 (1992), "Cloning of a Second Type of Activin Receptor and Functional Characterization in Xenopus Embryos".
Attisano et al., Cell, 68:97-108 (1992), "Novel Activin Receptors: Distinct Genes and Alternative mRNA Splicing Generate a Repertoire of Serine/Threonine Kinase Receptors".
Current Protocols in Molecular Biology, John Wiley & Sons, 1987, Chapter 11.
Mathews and Vale, Cell, 65:973-982 (1991), "Expression Cloning of an Activin Receptor, a Predicted Transmembrane Serine Kinase".
Massague, J. Biol. Chem., 260(11): 7059-7066 (1985), "Subunit Structure of a High-Affinity Receptor for Type .beta.-Transforming Growth Factor".
Cheifitz et al., J. Biol. Chem., 263(33):17225-17228 (1988), "A Surface Component of GH3 Pituitary Cells That Recognizes Transforming Growth Factor-.beta., Activin, and Inhibin".
Sporn and Roberts, Nature, 313:745-747 (1985), "Autocrine Growth Factors and Cancer".
Keller et al., J. Cell. Biochem., 39:175-184 (1989), "Transforming Growth Factor .beta.: Possible Roles in the Regulation of Normal and Leukemic Hematopoietic Cell Growth".
Kimichi et al., Science, 240:196-199 (1988), "Absence of TGF-.beta. Receptors and Growth Inhibitory Responses in Retinoblastoma Cells".
Kehrl et al., K. Immunol., 143(6):1868-1874 (1989), "Further Studies of the Role of Transforming Growth Factor-.beta. in Human B Cell Function".
Segarini et al., Mol. Endocrin., 3:261-272 (1989), "Binding of Transforming Growth Factor-.beta. to Cell Surface Proteins Varies with Cell Type".
Jennings et al., J. Cell. Physiol., 137:167-172 (1988), "Comparison of the Biological Action of TGF Beta-1 and TGF Beta-2: Differential Activity in Endothelial Cells".
Cheifetz et al., J. Biol. Chem., 263(22):10783-10789 (1988), "Heterodimeric Transforming Growth Factor .beta.".
Segarini and Seyedin, J. Biol. Chem., 263(17):8366-8370 (1988), "The High Molecular Weight Receptor to Transforming Growth Factor-.beta. Contains Glycosaminoglycan Chains".
Cheifetz and Massague, J. Biol. Chem., 261(21):9972-9978 (1986), "Cellular Distribution of Type I and Type II Receptors for Transforming Growth Factor-.beta.".
Tucker et al., PNAS USA, 81:6757-6761 (1984), "Specific Binding to Cultured Cells of 125 I-Labeled Type .beta. Transforming Growth Factor from Human Platelets".
Frolik, J. Biol. Chem., 259(17):10995-11000 (1984), "Characterization of a Membrane Receptor for Transforming Growth Factor-.beta. in Normal Rat Kidney Fibroblasts".
Cheifetz et al., J. Biol. Chem., 263(32):16984-16991 (1988), "The Transforming Growth Factor-.beta. Receptor Type III is a Membrane Proteoglycan".
Segarini et al., J. Biol. Chem., 262(30):14655-14662 (1987), "Membrane Binding Characteristics of Two Forms of Transforming Growth Factor-.beta.".
Cheifetz et al., Cell, 48:409-415 (1987), "The Transforming Growth Factor-.beta. System, a Complex Pattern of Cross-Reactive Ligands and Receptors".
Wakefield et al., J. Cell Biol., 105:965-975 (1987), "Distribution and Modulation of the Cellular Receptor for Transforming Growth Factor-Beta".
Massague and Like, J. Biol. Chem., 260(5):2636-2645 (1985), "Cellular Receptors for Type .beta. Transforming Growth Factor".
Mathews et al., 73rd Annual of the Endocrine Society, Jun. 19-22, 1991, Washington, D.C., abst. #1516, "Characterization of Activin A Receptors by Chemical Cross-Linking".
Cox Edward T.
Mather Jennie P.
Sliwkowski Mary B.
Woodruff Teresa K.
Adams Arnold E.
Chan Y. Christina
Genentech Inc.
Hasak Janet E.
LandOfFree
Detection and purification of activin polypeptide does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Detection and purification of activin polypeptide, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Detection and purification of activin polypeptide will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1206447