Detection and/or quantification method of target molecules...

Details Detection and/or quantification method of target molecules... Detection and/or quantification method of target molecules...

2011-08-09

2011-08-09

Kim, Young J (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091200

Reexamination Certificate

active

07993840

ABSTRACT:
The present invention relates to a method and device for detecting and/or quantifying one or multiple target molecules present in a solution by quantifying online their binding on specific capture molecules immobilized at different locations (spots) of a surface of an optically transparent solid support without substantial detection of target molecules present in solution. The present invention allows multiple target assays to be performed in a simultaneous detection. More particularly, the invention comprises detecting in real-time the hybridization between capture DNA molecules present on a micro-array and target polynucleotides present in solution. The invention is also related to real-time PCR of multiple targets on a micro-array.

REFERENCES:
patent: WO 9320240 (1993-10-01), None
patent: WO 03023377 (2003-03-01), None
patent: WO2006135437 (2006-12-01), None
Lehr et al. Real-Time Detection of Nucleic Acid Interactions by Total Internal Reflection Fluorescence. Analytical Chemistry (2003) 75: 2414-2420.
von Nickisch-Rosenegk et al. On-chip PCR amplification of very long templates using immobilized primers on glassy surfaces. Biosensors and Bioelectronics (2005) 20: 1491-1498.

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