Detection and/or identification of microorganisms in a test samp

Details Detection and/or identification of microorganisms in a test samp Detection and/or identification of microorganisms in a test samp

1985-05-31

1989-08-29

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 5, 435 8, 435 14, 435 18, 435 19, 435 21, 435 25, 435 26, 435 29, 435 32, 435 34, 435 36, 435 38, 435170, 4351721, 435261, 435822, 935 52, 935 55, 935 56, 935 57, 935 58, 935 79, 935 80, 935 82, C12Q 168, C12Q 166, C12Q 102, C12Q 104

Patent

active

048617091

ABSTRACT:
A method for determining the presence of microorganisms in a tests sample. Exogenous DNA containing a luminescent system or other genetic marker system derived from a suitable donor source is introduced by genetic means into a host microorganism which lacks or poorly expresses the donor DNA and whose presence it is desired to detect. Expression of the donor gene system allows the detection of the host microorganism. Compositions of bacteriophages and plasmids as well as a method for detection of antibiotics in a test sample are described.

REFERENCES:
patent: 4038143 (1977-07-01), Juni
patent: 4540667 (1985-09-01), Orser et al.
patent: 4581335 (1986-04-01), Baldwin
Engebrecht, J. et al., Cell, 32: 773-781 (1983).
Belas, R., et al Science 218: 791-793 (1982).
Maniatis, T. in "Molecular Cloning a Laboratory Manual", (Cold Spring Harbor publishers, New York) pp. 17-25 and 38-39 (1982).

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