Detection and measurement of circulating fibrin

Details Detection and measurement of circulating fibrin Detection and measurement of circulating fibrin

1978-11-20

1981-01-13

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving blood clotting factor

435 15, 435 23, C12Q 156, C12Q 148, C12Q 138

Patent

active

042450403

ABSTRACT:
An improved method is described for the detection and quantitation of Circulating Plasma Fibrin, also known as Soluble Fibrin (Fs), which is based upon the specific enzymatic transfer by Factor XIII, or Papain, of a Carbon-14 label in Glycine Ethyl Ester to Fs and which is improved upon by (a) substitution of the enzyme reaction in whole plasma for the enzyme reaction in an alcohol fraction of plasma, (b) inclusion of a serum blank to correct for low level non-specific labeling of plasma protein other than Fs, and (c) substitution of molecular, or gel, filtration for purification of Fs by chemical fractionation. The preceding improvements reduce the operational time for the assay from 72 hours to 4 hours, and reduce the labor time for the assay from 12 hours to 1 hour.

REFERENCES:
patent: 3294641 (1966-12-01), Lorand
Kisker et al., J. Clin. Invest., 50, (1971), 2235-2241.
Buluk et al., Chemical Abstracts 68: 47578p, (1968), p. 4593.

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