Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1984-07-19
1987-06-23
Nucker, Christine M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 18, 435 91, 435803, 436501, 424 11, 2041828, 935 76, 935 77, 935 78, C12Q 168
Patent
active
046752838
ABSTRACT:
A novel method for detecting and isolating DNA sequences commonly held by different DNA preparations or repeated or amplified within a complex genome has been provided. The DNA preparations of interest are digested with the same restriction enzyme and a portion of at least one preparation is labeled with .sup.32 P. The labeled and unlabeled DNA preparations are combined and electrophoresed in an agarose gel. Following electrophoresis, the DNA is denatured in situ and allowed to reanneal within the gel so that homologous DNA sequences present within restriction fragments of the same size can reanneal. After reannealing, unhybridized single-stranded DNA is digested in situ followed by detection of the reannealed DNA by autoradiography. When labeled and unlabeled DNAs are derived from different DNA preparations, only the restriction fragments commonly held by these two preparations are detected. When a restriction digest of total eukaryotic DNA is reassociated in the gel by this procedure, repeated restriction fragments are selectively detected. This approach permits detection of selectively amplified DNA sequences and identification of the DNA sequences that have been commonly amplified in different cell populations. Localized of homologous, repeated or amplified DNA fragments of interest within the gel permits size-purification of such fragments.
REFERENCES:
patent: 4358535 (1982-11-01), Falkow et al.
patent: 4395486 (1983-07-01), Wilson et al.
patent: 4399216 (1983-08-01), Axel et al.
patent: 4532220 (1985-07-01), Lavi
Cook Paul J.
Massachusetts Institute of Technology
Nucker Christine M.
Wieder Stephen C.
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