Detection and estimation of microquantities of alkylating agents

Details Detection and estimation of microquantities of alkylating agents Detection and estimation of microquantities of alkylating agents

1970-06-04

1978-04-11

Schafer, Richard E.

Chemistry: physical processes

Physical processes

Crystallization

23230R, 252408, 260924, 260934, G01N 3122

Patent

active

040836924

ABSTRACT:
A method comprising an unknown solution adding an organophosphorus thio a salt solution for sufficient time to form compounds having anticholinesterase activity in the reaction solution, contacting the reaction solution with a cholinesterase supported on a suitable substrate, adding indoxyl acetate and observing a non-color or blue color change in the substrate; the absence of color indicates the formation of compounds with anticholinesterase activity and the blue color of the substrate indicating that there was no formation of compounds with anticholinesterase activity.

REFERENCES:
patent: 2911430 (1959-11-01), Fitch
patent: 2919977 (1960-01-01), Grant
patent: 2980581 (1961-04-01), Schrader
patent: 3049411 (1962-08-01), Gelman et al.
patent: 3082240 (1963-03-01), Lorenz et al.
patent: 3309371 (1967-03-01), Curry et al.
patent: 3451901 (1969-06-01), Seiger et al.

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