Detection and differentiation of coxiella burnetii in biological

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 34, 435 35, 4351723, 436 63, 436501, 536 27, 935 29, 935 78, C12Q 168, C07H 1512

Patent

active

048761869

ABSTRACT:
Methods for detecting the presence of Coxiella burnetii in biological samples, as well as a method for differentiating strains of C. burnetii that are capable of causing acute disease from those strains capable of causing chronic disease are disclosed. The methods generally comprise treating cells contained within the biological sample to expose cellular DNA, and hybridizing the cellular DNA (specifically rickettsial DNA) with a C. burnetii-specific labeled DNA probe. Radioisotope and biotin labels are preferred, allowing detection through autoradiography and colorimetric assays, respectively.

REFERENCES:
patent: 4332898 (1982-06-01), Reusser
patent: 4358535 (1982-11-01), Falkow et al.
patent: 4563419 (1986-01-01), Rank et al.
O. G. Baca and D. Paretsky, "Q Fever and Coxiella burnetii: A Model for Host-Parasite Interactions", Microbial Rev. 47: 127-149, 1983.
R. A. Ormsbee, Q Fever Rickettsia, in "Viral and Rickettsial Infections of Man", 4th Ed., F. L. Horsfall, Jr. and I. Tamm (ed.), J. B. Lippincott Co., PA, 1965, pp. 1144-1160.
M. G. Peacock et al., "Serological Evaluation of Q Fever in Humans: Enhanced Phase I Titers of Immunoglobulins G and A are Diagnostic for Q Fever Endocarditis", Infect. Immun. 41: 1089-1098, 1983.
W. P. G. Turck et al., "Chronic Q Fever", Q. J. Med. 45: 193-217, 1976.
M. J. Tobin et al., "Q Fever Endocarditis", Am. J. Med. 72: 396-400, 1982.
A. O. Robson and C. D. G. L. Shimmin, "Chronic Q Fever-I. Clinical Aspects of a Patient with Endocarditis", Br. Med. J. 2:980-983, 1959.
M. G. P. Stoker and P. Fiset, "Phase Variation of the Nine Mile and Other Strains of Rickettsia burnetii", Can J. Microbiol. 2: 310-321, 1956.
J. E. Samuel et al., "Isolation and Characterization of a Plasmid from Phase I Coxiella burnetii", Infect. Immun. 41: 488-493, 1983.
L. P. Mallavia et al., "Coxiella burnetii Plasmid DNA, in Microbiology 1984", L. Leive and D. Schlessinger (ed.), Amer. Soc. Microbiol., Washington, D.C. 1984, pp. 293-296.
J. E. Samuel et al., "Correlation of Plasmid Type and Disease Caused by Coxiella burnetii", Infect. Immun. 49: 775-779, 1985.
J. J. Leary et al., "Rapid and Sensitive Colorimetric Method for Visualizing Biotin-Labeled DNA Probes Hybridized to DNA or RNA Immobilized on Nitrocellulose: Bio-Blots", Proc. Natl. Acad. Sci. USA 80: 4045-4049, 1983.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Detection and differentiation of coxiella burnetii in biological does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Detection and differentiation of coxiella burnetii in biological, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Detection and differentiation of coxiella burnetii in biological will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1588832

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.