Detection and amplification of ligands

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S007900, C435S007200, C435S069100, C435S005000, C349S123000, C349S124000, C349S130000, C349S137000, C530S350000, C536S023400, C536S023500, C436S004000, C436S501000, C436S524000, C436S528000

Reexamination Certificate

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10133610

ABSTRACT:
A system for the detection of ligands comprising at least one receptor and an amplification mechanism coupled to the receptor wherein an amplified signal is produced as a result of receptor binding a ligand. Examples of suitable amplification mechanisms include antibody-embedded liquid crystalline materials; use of alpha-2-macroglobulin to encage an enzyme, whereby the enzyme is separated from its substrate by an receptor; and a receptor engineered to inhibit the active of site of an enzyme only in the absence of a ligand. Also provided are methods for the automatic detection of ligands.

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