Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1997-03-18
2000-11-14
Minnifield, Nita
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 722, 435 792, 435961, 435968, 435973, 435975, 435971, 436526, 436540, 436177, G01N 3353, G01N 33531, G01N 33553, G01N 33541
Patent
active
06146838&
ABSTRACT:
A method for the detection or quantitation of a water-borne parasite, such as Cryptosporidia. The detection or quantitation is accomplished by an electrochemiluminescence assay comprising the steps of filtering water to obtain a sludge thought to contain the parasite or a fragment thereof; extracting a sample of said sludge in a extraction medium to form antigenic derivatives of said parasite; forming an assay mixture comprising a sample of said extracted sludge and an antibody specific to said antigenic derivative; incubating said assay mixture to permit binding of said antibody and said antigenic derivative; and conducting an electrochemiluminescence assay for the bound complex of antibody and antigenic derivative, thereby detecting or quantitating the Cryptosporidia in said water.
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Robertson et al., "In Vitro Excystation of Cryptosporidium parvum," Parasitology, 106 (Part 1):13-19, Jan. 1993.
Campbell et al., "Detection of Oocysts of Cryptosporidium by Enhanced Chemiluminescence," J. of Microbiol Methods, 17: 297-303, 1993.
Johnson et al., "Quantitative Assay Development for Cryptosporidium" FASEB Journal, 9 (3 Part I): A229, Abstract No. 1328, Mar. 9, 1995.
Johnson et al., "Development of a PCR Protocol for Sensitive Detection of Cryptosporidium Oocysts in Water Samples," Applied Environ. Microbiol. 61(11): 3849-3855, Nov. 1995.
Jakubowski et al., "Environmental Methods for Cryptosporidium," American Water Works Association Journal. 88(9):107-121, 1996.
Kenten John H.
Williams Richard O.
Baskar Padma
IGEN International Inc.
Minnifield Nita
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