Detecting the expression of the DESC1 gene in squamous cell...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C536S023100, C536S024310

Reexamination Certificate

active

09674035

ABSTRACT:
The present invention provides a novel method for diagnosing squamous cell carcinoma or prostate cancer in a tissue sample. The method does not involve visual examination of morphology. The method comprises providing a sample from the subject and assaying for the presence, or absence or reduced level of expression of a novel gene, hereinafter referred to as the “DESC1 gene”. The method comprises isolating RNA, preferably mRNA from tissue samples, and detecting the mRNA which encodes all or part of DESC1 protein. Preferably the detection comprises amplifying the mRNA, preferably by reverse transcriptase-PCR using primers specific to a region in the DESC1 gene; and detecting the presence or absence of the amplified product to determine whether DESC1 mRNA is present or absent in the tissue sample. Alternatively the detection comprises separating the RNA which encodes all or part of DESC1 protein from the total RNA, preferably by separating on an agarose gel, and detecting the mRNA encoding DESC1, preferably by using a probe specific for such mRNA. Optionally, the DESC1 mRNA when present, is also quantified using conventional techniques. The present invention also relates to polynucleotides which encode the DESC1 protein, to the DESC1 protein, and to antibodies to the DESC1 protein. The present invention also relates to hybridization probes, and to primers useful in the method of detecting DESC1 mRNA.

REFERENCES:
patent: 2003/0073129 (2003-04-01), Baker et al.
patent: 00/12708 (2000-03-01), None
Reiger et al. (Glossary of Genetics and Cytogenetics, Classical and Molecular, 4th Ed., Springer-Verlay, Berlin, 1976.
Genes IV (Lewin et al, Oxford University Press, p. 810, 1990.
Burgess et al, Journal of Cell Biology vol. 111 Nov. 1990 2129-2138.
Lazar et al Molecular and Cellular Biology Mar. 1988 vol. 8 No. 3 1247-1252.
Schwartz et al, Proc Natl Acad Sci USA vol. 84:6408-6411 (1987.
Lin et al Biochemistry USA vol. 14:1559-1563 (1975.
The 1991 Boehringer Mannheim Biochemical Catalog, p. 557.
Abstract No. 344, “Gene Alterations in Squamous Cell Carcinoma of the Head and Neck” by Lang, et al.International Journal of Molecular Medicine,vol. 6, Supplement 1, Proceedings of the Abstracts of the 5 World Congress on Advances in Oncology and 3rdInternational Symposium on Molecular Medicine, Oct. 19-21, 2000, Hersonissos, Crete, Greece.
Abstract No. 194, “Isolation of Differently Expressed Genes in Squamous Cell Carcinoma of the Head and Neck” by Lang, et al., 5thInternational Conference on Head and Neck Cancer, Jul. 29-Aug. 2, 2000, San Francisco, California.
Abstract No. 30, “Differential Expression of a Novel Serine Protease-Like Gene in Squamous Cell Carcinoma of the Head and Neck” by Lang, et al., The Ohio State University Comprehensive Cancer Center Scientific Retreat, Cherry Valley Lodge, Newark, Ohio, Dec. 16, 1999.
Genbank Accession No. NM—041058, Nov. 2, 2000.
Genbank Accession No. AF064819, Oct 3, 2000.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Detecting the expression of the DESC1 gene in squamous cell... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Detecting the expression of the DESC1 gene in squamous cell..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Detecting the expression of the DESC1 gene in squamous cell... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3725195

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.