Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2007-03-27
2007-03-27
Riley, Jezia (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C005S007000, C005S007000
Reexamination Certificate
active
10396960
ABSTRACT:
This invention relates to agents and conjugates that can be used to detect and isolate target components from complex mixtures such as nucleic acids from biological samples, cells from bodily fluids, and nascent proteins from translation reactions. Agents comprise a detectable moiety bound to a photoreactive moiety. Conjugates comprise agents coupled to substrates by covalent bounds which can be selectively cleaved with the administration of electromagnetic radiation. Targets substances labeled with detectable molecules can be easily identified and separated from a heterologous mixture of substances. Exposure of the conjugate to radiation releases the target in a functional form and completely unaltered. Using photocleavable molecular precursors as the conjugates, label can be incorporated into macromolecules, the nascent macromolecules isolated and the label completely removed. The invention also relates to targets isolated with these conjugates which may be useful as pharmaceutical agents or compositions that can be administered to humans and other mammals. Useful compositions include biological agents such as nucleic acids, proteins, lipids and cytokines. Conjugates can also be used to monitor the pathway and half-life of pharmaceutical composition in vivo and for diagnostic, therapeutic and prophylactic purposes. The invention also relates to kits comprised of agents and conjugates that can be used for the detection of diseases, disorders and nearly any individual substance in a complex background of substances.
REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 5043272 (1991-08-01), Hartley
patent: 5057410 (1991-10-01), Kawasaki et al.
patent: 5106727 (1992-04-01), Hartley et al.
patent: 5252743 (1993-10-01), Barrett et al.
Thiele et al. Photocleavable Biotinylated Ligands for Affinity Chromatography, Analytical Biochemistry, vol. 218, pp. 330-337, 1994.
Adler-Storthz, et al., “Biotin-Avidin-Amplified Enzyme Immunoassay for Detection of Herpes Simplex Virus Antigen in Clinical Specimens,”Journal of Clinical Microbiology18: 1329-1334 (1983).
Baldwin, et al., “New Photolabile Phosphate Protecting Groups,”Tetrahedron46:6879-6884 (1990).
Bayer, et al., “Affinity Cytochemistry: The Localization of Lectin and Antibody Receptors on Erythrocytes Via the Avidin-Biotin Complex,”FEBS Letters68: 240-244 (1976).
Beard, et al., “Identification of Monoclonal Antibodies Against the Trypomastigote Stage ofTrypanosoma Cruziby Use of Iminobiotinylated Surface Polypeptides,”Molecular and Biochemical Parasitology16: 199-212 (1985).
Bednarek and Bodanszky. “9-Fluorenylmethyl Esters”Int. J. Peptide Protein Res. 21:196-201 (1983).
Berenson et al., “Positive selection of viable cell populations using avidin-biotin immunoadsorption,”J. Immunol. Methods91:11-19 (1986).
Bradford, “A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding,”Analytical Biochemistry72: 248-254 (1976).
Brigati, et al., “Detection of Viral Genomes in Cultured Cells and Paraffin-Embedded Tissue Sections Using Biotin-Labeled Hybridization Probes,”Virology126:32-50 (1983).
Broker, et al., “Electron Microscopic Visualization of tRNA Genes With Ferritin-Avidin: Biotin Labels,”Nucleic Acids Research5:363-384 (1978).
Buckie and Cook, “Specific Isolation of Surface Glycoproteins from Intact Cells by Biotinylated Concanavalin A and Immobilized Streptavidin,”Analytial Biochemistry156: 463-472 (1986).
Casali, et al. “Human Monoclonals from Antigen-Specific Selection of B Lymphocytes and Transformation by EBV,”Science234:476-479.
Chandler and Ballard, “Multiple Biotin-Containing Proteins in 3T3-L1 Cells,”Biochem. J. 237:123-130 (1986).
Delius, et al., “Separation of Complementary Strands of Plasmid DNA Using the Biotin-Avidin System and ots Application to Heteroduplex Formation and RNA/DNA Hybridization in Electron Microscopy,”Nucleic Acids Research13:5457-5469 (1985).
Diamandis and Christopoulos, “The Biotin-(Strept) Avidin System: Principles and Applications in Biotechnology,”Clin. Chem. 37/5:625-636 (1991).
DiCesare et al., “A High-Sensitivity Electrochemiluminescence-Based Detection System for Automated PCR Product Quantitation,”BioTechniques15:152-59 (1993).
Duhamel and Johnson, “Use of Nonfat Dru Milk to Block Nonspecific Nuclear and Membrane Staining by Avidin Conjugates,”The Jounral of Histochemistry and Cytochemistry33:711-714 (1985).
Eckermann, et al., “Sequence at the Site of Attachment of an Affinity-Labeled Derivative of Puromycin on 23-S Ribosomal RNA ofEscherichia coliRibosomes,”Eur. J. Biochem. 82: 225-234 (1978).
Elbe, et al. “Fetal Skin: A Site of Dendritic Epidermal T Cell Development,”The Journal of Immunology149:1694-1701 (1992).
Esteban, et al., “An Amplified Immunoperoxidase Assay to Detect Bovine Leukemia Virus Expression: Development and Comparison with Other Assays,”Cancer Research45:3231-3235 (1985).
Finn, et al., “Avidin-Biotiin Affinity Chromatorgraphy:Application to the Isolation of Human Placental Insulin Receptor,”Proc.Natl.Acad. Sci. USA81:7328-7332 (1984).
Finn and Hofmann, “Isolation and Characterization of Hormone Receptors,”Methods Biochem. Anal. 26:244-274 (1980).
Gebeyhu, et al., “Novel Biotinylated Nucleotide- Analogs for Labeling and Colorimetric Detection of DNA,”Nucleic Acids Research15:4513-4534 (1987).
Gould, et al., “Use of the Biotin-Streptavidin Interaction To Improve Flavivirus Detection By Immunofluorescence and Elisa Tests,”Journal of Virological Methods11:41-48 (1985).
Grabowski and Sharp, “Affinity Chromatography of Splicing Complexes: U2, U% and U4+U6 Small Nuclear Ribonucleoprotein Particles in the Spliceosome,”Science233: 1294-1299 (1986).
Guerin-Reverchon, et al., “A Comparison of MEthods for the Detection of Human Papillomavirus DNA by in situ Hybridization with Biotinylated Proves on Human Carcinoma Cell Lines,”Journal of Immunological Methods123:167-176 (1989).
Happ et al., “New Approach to the Synthesis of 2′(3′)-O-Aminoacyl Oligoribonucleotides,”J. Org. Chem. 52:5387-91 (1987).
Hazum. “Purification of Gonadotropin-Releasing Hormone Receptors,”Methods in Enzymology184:285-293 (1990).
Heckler et al., “Preparation of 2′(3′)-O-Acyl-pCpA Derivatives as Substrates for T4 RNA Ligase-Mediated “Chemical Aminoacylation”,”Tetrahedron40:87-94 (1984).
Hudson, “Methodological Implications of Simultaneous Solid-Phase Peptide Synthesis. 1. Comparison of Different Coupling Procedures,”J. Org. Chem. 53:617-624 (1988).
Ito, et al., “Triplex Affinity Capture of a Single Copy Clone From a Yeast Genomic Library,”Nucleic Acids Research20:3524 (1992).
Johnson, et al., “Functional Covalent Complez Between Elongation Factor Tu and an Analog of Lysyl-tRNA,”Proc. Natl. Acad. Sci. USA75:3075-3079 (1978).
Jones, et al., “Elimination of the Non-specific Binding of Avidin to Tissue Sections,”Histochemical Journal19:264-268 (1987).
Kendall, et al., “Utilization of the Biotin/Avidin System to Amplify the Sensitivity of the Enzyme-Linked Immunosorbent Assay (ELISA),”Journal of Immunological MEthods56:329-339 (1983).
Khorana, “Bacteriorhodopsin, a Membrane Protein That Uses Light to Translocate Protons,”The Journal of Biological Chemistry263:7439-7442 (1988).
Kohanski and Lane. “Homogeneous Functional Insulin Receptor from 3T3-L1 Adipocytes.”The Journal of Biological Chemistry260: 5014-5025 (1985).
Krieg et al., “Photocrosslinking of the signal sequence of nascent preprolactin to the 54-kilodalton polypeptide of the signal recognition particle,”Proc. Natl. Acad. Sci. USA83:8604-08 (1986
Olejnik Jerzy
Rothschild Kenneth J.
Sonar Sanjay M.
Medlen & Carroll LLP
Riley Jezia
The Trustees of Boston University
LandOfFree
Detecting targets in heterologous mixtures does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Detecting targets in heterologous mixtures, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Detecting targets in heterologous mixtures will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3771932