Detecting polynucleotide sequences

Details Detecting polynucleotide sequences Detecting polynucleotide sequences

1985-02-28

1987-12-29

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 5, 436501, 536 26, 536 27, 536 28, 935 77, 935 78, C12Q 170, C12Q 168, G01N 33566

Patent

active

047161060

ABSTRACT:
A method of detecting a target polynucleotide sequence in a sample uses a labelled polynucleotide secondary probe, and a polynucleotide primary probe having sequences complementary to both the target and the secondary probe. The sample, immobilized or in solution is hybridized with the primary probe; and the labelled secondary probe is also hybridized with the primary probe. The method permits the production of a labelled secondary probe which can be used in conjuction with many different primary probes for different hybridization reactions.

REFERENCES:
Dunn, A. R. et al., Methods in Enzymology, 65: 468-478, (1980).

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