Detecting or quantifying substances using labelling techniques

Details Detecting or quantifying substances using labelling techniques Detecting or quantifying substances using labelling techniques

1978-11-14

1984-10-23

Meyers, Albert T.

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

Molecular bilayer structure

436547, 436800, 435 7, G01N 3100, G01N 3122, G01N 3348, G01N 3354, G01N 3356

Patent

active

044788177

ABSTRACT:
The invention relates to a method of detecting, analyzing, quantifying or locating a protein, antibody, antigen, hapten, hormone, metabolite, nucleic acid or steroid, in which the substance of interest is linked to a chemi-luminescent or bio-luminescent label. A luminescent reaction is then triggered by the addition of an oxidizing agent or a catalyst and the emitted light is observed in order to provide information about the substance. The invention employs a luminescent reagent which consists of antibodies labelled with a luminescent material such as luminol. The luminescent reagent can be used to quantify antigens in an immunological assay. The luminescent labelled antibodies selectively bind to the antigens and the amount of light emitted in a luminescent reaction gives an indication of the amount of antigens present. A luminescent labelled substance can also be reacted with an antibody or antigen labelled with a fluorescent material in order to carry out a homogeneous assay.

REFERENCES:
patent: 3992631 (1976-11-01), Hartz
patent: 4000252 (1976-12-01), Kosak
patent: 4025310 (1977-05-01), Bolz
patent: 4036946 (1977-07-01), Kleinerman
patent: 4041146 (1977-08-01), Giaever
patent: 4104029 (1978-08-01), Maier
patent: 4128628 (1978-12-01), Brooker
patent: 4153675 (1979-05-01), Kleinerman
patent: 4160645 (1979-07-01), Ullman
patent: 4172827 (1979-10-01), Giaever
patent: 4208479 (1980-06-01), Zuk
Chappelle, Biochem. Med., vol. 2, 1968 pp. 41-52.

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