Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1992-04-16
1993-08-03
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 34, 435287, 435291, 435296, C12Q 104, C12Q 106, C12M 134, C12M 124
Patent
active
052328392
ABSTRACT:
In such a method and apparatus for detecting microbiological growth in a sealed sample container, pressure within the headspace of the container is monitored for detecting rate of change of such headspace pressure. Presence of microbiological growth within the container is indicated as a function of rate of change of headspace pressure. The rate of pressure change is compared with a standard rate for a family of microorganisms., and growth is indicated when the absolute value of the rate of pressure change (which may be positive or negative) exceeds the absolute value of the standard rate (which also may be positive or negative). For detecting microbiological growths which may have differing growth rates, absolute value of rate of pressure change is compared to a plurality of standard rates for differing families of microorganisms. At least some of the rates may be positive, corresponding to growth of gas-producing organisms, and at least some of the rates may be negative, corresponding to growth of gas-consuming organisms. In either case, microbiological growth in the test sample is indicated when absolute mathematical value of the rate of headspace pressure change exceeds absolute mathematical value of any of the standard rates.
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Eden Gideon
Sullivan Nadine M.
Difco Laboratories Incorporated
Trembley T. A.
Warden Robert J.
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