Detecting isomers using differential derivatization mass...

Details Detecting isomers using differential derivatization mass... Detecting isomers using differential derivatization mass...

2011-04-12

2011-04-12

Gakh, Yelena G (Department: 1777)

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

Amino acid or sequencing procedure

C422S067000

Reexamination Certificate

active

07923257

ABSTRACT:
Methods of evaluating molecular isomers of branched-chain amino acids are featured. The methods can include: derivatizing one or more molecular isomers of branched-chain amino acids in a sample comprising a branched-chain amino acid labeled with one or more heavy atoms as a first standard; adding, to the sample, after derivatization, a nonderivatized or derivatized branched chain amino acid that is labeled with one or more heavy atoms, as a second standard; evaluating the sample using tandem mass spectrometry; and detecting peaks indicative of derivatized and nonderivatized forms of one or more branched-chain amino acids in the sample.

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