Detecting eubacteria and fungus and determining their antibiotic

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

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435 18, 435 29, 435 34, 435206, C12Q 118, C12Q 134, C12Q 102, C12Q 104, C12N 936

Patent

active

060905735

ABSTRACT:
A method is presented for detecting eubacteria and fungus in biological samples, and, if present, determining their antibiotic sensitivity, where the presence and amount of eubacteria or fungus is measured by means of catalytically inactive murein binding enzymes in both parts of the method. The sensitivity of eubacteria or fungus to a test antibiotic is obtained by comparing the amount of eubacteria or fungus in the sample after culturing the sample in the presence and absence of the test antibiotic. The biological sample may be chemically treated with alkali to cleave peptide bonds in the sample before incubating the sample with the catalytically inactive murein binding enzyme.

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