Detecting environmental pollutants with protoplasts in alginate

Details Detecting environmental pollutants with protoplasts in alginate Detecting environmental pollutants with protoplasts in alginate

1984-07-24

1988-01-19

Marantz, Sidney

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

422 57, 436 34, 436 39, 436 63, 436 82, 436141, G01N 3318

Patent

active

047204532

ABSTRACT:
Plant protoplasts are suspended in a solution of sodium alginate which is thereafter thickened by the addition of a soluble calcium or lanthanum salt to produce a matrix with large pores permeable to water and gas in which the protoplasts are held. The resulting material, in tape or spheroid form, when stored in a nutrient solution, will prolong the senescence of the protoplasts and increase their sensitivity to polluting materials. When such ribbons or particles are exposed to a polluting environment for a particular period of time, the effect of pollution can be detected by measuring the evolution of ethane or measuring the blocking of the enzyme ribulose-diphosphate-carboxylase, in the latter case using carbon dioxide marked with .sup.14 C. A control experiment in which an identical indicator treated in an equivalent environment without pollutants reveals, by comparison, the effect of the pollution.

REFERENCES:
Chemical Abstracts, I, 94:162739r (1981).
Chemical Abstracts, II, 99:119537q (1983).
Siegel, "Water Air Soil Pollut., 8(3), 1977, pp. 293-304 (Abstract).
Misaghi, Physiology and Biochemistry of Plant-Pathogen Interactions, Plenum Press, N.Y. (1982), pp. 86-87.

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