Detecting and amplifying target nucleic acids using exonucleolyt

Details Detecting and amplifying target nucleic acids using exonucleolyt Detecting and amplifying target nucleic acids using exonucleolyt

1993-08-04

1996-11-12

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 9151, 435 9152, 435 9131, 536 243, C12Q 168, C12P 1934, C07H 2104

Patent

active

055739075

ABSTRACT:
The present invention relates to improved LCR amplification schemes using at least one downstream probe modified at its 5' end to reduce or eliminate target independent amplification. The different modified probes, and kits containing them are also presented. Also presented is a method for detecting differences in nucleic acid sequences, with reduced target independent amplification, using the modified probes.

REFERENCES:
patent: 4851331 (1989-07-01), Vary et al.
Murakami et al Nucleic Acid Research 1991 19: 4097-4102.
Howard et al Proc Natl Acad Sci, USA Biochemistry (1991) 88: 7276-7280.
Habraken et al., "Chromatin 3'-phosphatase/5'-OH kinase cannot transfer phosphate from 3' to 5' across a strand nick in DNA", Nucleic Acids Research, vol. 14, No. 20, issued 1986.
Brutlag et al., "Enzymatic Synthesis of Deoxyribonucleic Acid", J. Biol. Chem., vol. 247, No. 1, pp. 241-248, issued 10 Jan. 1972.
Wu et al., "The ligation amplification reaction (LAR)-amplification of specific DNA sequences using sequential rounds of template-dependent ligation", Genomics 4. pp. 560-569 (1989).
Bailey et al., Biochem. J., 259 (1989) pp. 761-768.

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