Design and synthesis of biotinylated probes for N...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

Reexamination Certificate

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C548S304400, C549S033000

Reexamination Certificate

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07955816

ABSTRACT:
The present invention relates to the synthesis and characterization of biotinylated analogue of N-arachidonoylethanolamine (AEA) and its use as a tool to study AEA transport and trafficking through biochemical and morphological techniques. In particular biotinylated AEA (b-AEA, for which we propose the common name MM22) is suitable to design highly sensitive and simple methods for the non-radioactive detection and quantitation of AEA from complex samples, which would offer a useful alternative approach to the routinely used radiometric assays. The invention also relates to the use of b-AEA as a potential therapeutic and diagnostic agent.

REFERENCES:
patent: 6358682 (2002-03-01), Jaffee et al.
patent: 2005/085873 (2005-10-01), None
McReynolds et al., Bioorganic & Medicinal Chemistry vol. 10 (2002), p. 625-637.
Zhang et al. Bioorganic & Medicinal Chemistry, vol. 10 (2002), p. 2635-2639.
Greg T. Hermanson, Bioconjugate Techniques, Chapters 13 and 14, (1997).
K.D. McReynolds et al. “Non-natural glycosphingolipids and structurally simpler analogues bind HIV-1 recombinant gp120” Bioorganic & Medicinal Chemistry, vol. 10, No. 3, pp. 625-637 (2002).
Y. Zhang et al. “Detection of protein-ligand interaction on the membranes using C-terminus biotin-tagged alamethicin” Bioorganic & Medicinal Chemistry, vol. 10, No. 8, pp. 2635-2639 (2002).
Int'l Search Report for PCT/EP2006/061988, six pages (Aug. 7, 2007).
Written Opinion for PCT/EP2006/061988, eight pages (Aug. 7, 2007).

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