Desaturase genes and uses thereof

Details Desaturase genes and uses thereof Desaturase genes and uses thereof

2006-06-27

2006-06-27

Guzo, David (Department: 1636)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using a micro-organism to make a protein or polypeptide

C435S325000, C435S320100, C435S254110, C435S189000, C536S023100, C536S023200, C800S008000, C424S093210

Reexamination Certificate

active

07067285

ABSTRACT:
The subject invention relates to the identification of genes involved in the desaturation of polyunsaturated fatty acids at carbon 5 (i.e., “Δ5-desaturase”) and at carbon 6 (i.e., “Δ6-desaturase”) and to uses thereof. In particular, Δ5-desaturase may be utilized, for example, in the conversion of dihomo-γ-linolenic acid (DGLA) to arachidonic acid (AA) and in the conversion of 20:4n-3 to eicosapentaenoic acid (EPA). Delta-6 desaturase may be used, for example, in the conversion of linoleic (LA) to γ-linolenic acid (GLA). AA or polyunsaturated fatty acids produced therefrom may be added to pharmaceutical compositions, nutritional compositions, animal feeds, as well as other products such as cosmetics.

REFERENCES:
patent: 4582788 (1986-04-01), Erlich
patent: 4666701 (1987-05-01), Horrobin et al.
patent: 4683194 (1987-07-01), Saiki et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4758592 (1988-07-01), Horrobin et al.
patent: 4826877 (1989-05-01), Stewart et al.
patent: 4943674 (1990-07-01), Houck et al.
patent: 4945050 (1990-07-01), Sanford et al.
patent: 5004863 (1991-04-01), Umbeck
patent: 5106739 (1992-04-01), Comai et al.
patent: 5107065 (1992-04-01), Shewmaker et al.
patent: 5116871 (1992-05-01), Horrobin et al.
patent: 5159135 (1992-10-01), Umbeck
patent: 5175095 (1992-12-01), Martineau et al.
patent: 5188958 (1993-02-01), Moloney et al.
patent: 5231020 (1993-07-01), Jorgensen et al.
patent: 5416011 (1995-05-01), Hinchee et al.
patent: 5420034 (1995-05-01), Kridl et al.
patent: 5447974 (1995-09-01), Peng
patent: 5463174 (1995-10-01), Moloney et al.
patent: 5518908 (1996-05-01), Corbin et al.
patent: 5552306 (1996-09-01), Thomas et al.
patent: 5569834 (1996-10-01), Hinchee et al.
patent: 5589379 (1996-12-01), Kridl et al.
patent: 5631152 (1997-05-01), Fry et al.
patent: 5700671 (1997-12-01), Prieto et al.
patent: 5750176 (1998-05-01), Prieto et al.
patent: 5912120 (1999-06-01), Goldstein et al.
patent: 5972664 (1999-10-01), Knutzon et al.
patent: 2002/0156254 (2002-10-01), Qiu et al.
patent: 050424 (1985-09-01), None
patent: 084796 (1990-05-01), None
patent: 201184 (1992-12-01), None
patent: 258017 (1997-04-01), None
patent: 237362 (1998-10-01), None
patent: 1035207 (2000-09-01), None
patent: WO 93/06712 (1993-04-01), None
patent: WO 93/11245 (1993-06-01), None
patent: WO 94/11516 (1994-05-01), None
patent: WO 95/24494 (1995-09-01), None
patent: WO 96/13591 (1996-05-01), None
patent: WO 99/61602 (1999-12-01), None
patent: WO 00/20603 (2000-04-01), None
patent: WO 00/0075341 (2000-12-01), None
patent: WO 02/26946 (2002-04-01), None
Kelder et al. Expression of fungal desaturase genes in cultured mammalian cells. Mol Cell Biochem. Mar. 2001;219(1-2):7-11.
Houdebine LM. Transgenic animal bioreactors. Transgenic Res. 2000;9(4-5):305-20.
Richards FM. Protein stability: still an unsolved problem. Cell Mol Life Sci. Oct. 1997;53(10):790-802.
Qiu, Xiao, et al., “Identification of a DELTA4 Fatty Acid Desaturase fromThraustochytriumsp. Involved in the Biosynthesis of Docosahexanoic Acid by Hetrologous Expression inSaccharomyces cerevisiaandBrassica juncea,”Journal of Biological Chemistry, vol. 276, No. 34, (Aug. 24, 2001) pp. 31561-31566.
Saito, Tamao and Ochia, Hiroshi, “Identification of DELTA5-Fatty Acid Desaturase from the Cellular Slime Mold Dictyostelium Discoideum,”Eur. J. Biochem., vol. 265, (1999), pp. 809-814.
Leonard, Amanda E., et al., “cDNA Cloning and Characterization of Human DELTA5-Desaturase Involved in the Biosynthesis of Arachidonic Acid,”Biochem J., vol. 347, (2000), pp. 719-724.
Cho, Hyekyung P., et al., “Cloning, Expression, and Fatty Acid Regulations of the Human DELTA-5 Desaturase,”The Journal of Biological Chemistry, vol 274, No. 52, (Dec. 24, 1999) pp. 37335-37339.
Sakuradani, Eiji, et al., “DELTA6-Fatty Acid Desaturase from and Arachidonic Acid-ProducingMortierellaFungus Gene Cloning and its Heterologous Expression in a Fungus,Aspergillus,”GENE: An International Journal on Genes and Genomes, vol. 238, No. 2, (1999), pp. 445-453.
Huang, Yung-Sheng, et al., “Cloning of DELTA12- and DELTA6-Desaturases fromMortierella alpinaand Recombinant Production of GAMMA-Linolenic Acid inSaccharomyces cerevisiae,”Lipids, vol. 34, No. 7, (Jul. 1999), pp. 649-659.
Alonso, D. Lopez, et al., “Plants as ‘Chemical Factories’ for the Production of Polyunsaturated Fatty Acids,”Biotechnology Advances, vol. 18, (2000), pp. 481-497.
The Faseb Journal, Abstracts, Part I, Abstract 3093, p. A532, Experimental Biology 98, San Francisco, CA (Apr. 18-22, 1998).
Altschul, et al.,Nucleic Acids Research, Gapped Blast and PSI-Blast: A New Generation of Protein Database Search Programs, 25: 3389-3402 (1997).
Okamuro & Goldberg,Biochemistry of Plants, Regulation of Plant Gene Expression: General Principles, 15:1-82 (1989).
Turner & Foster,Molecular Biotechnology, The Potential Exploitation of Plant Viral Translational Enhancers in Biotechnology for Increased Gene Expression, 3:225 (1995).
Ingelbrecht, et al.,Plant Cell, Different 3′ End Regions Strongly Influence the Level of Gene Expression in Plant Cells, 1:671-680 (1989).
Klein, et al,Nature(London), High-Velocity Microprojectiles for Delivering Nucleic Acids Into Living Cells, 327:70-73 (1987).
Ishida Y., et al.,Nature Biotech, High Efficiency Transformation of Maize (Zea maysL.) Mediated byAgrobacterium tumefaciens, 14:745-750 (1996).
Mulles, et al,Cold Spring Harbor Symp. Quant. Biol., Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain Reaction, 51:263-273 (1986).
Jones, et al.EMBO J., High Level Expression of Introduced Chimaeric Genes in Regenerated Trasformed Plants, 4:2411-2418 (1985).
DeAlmeida, et al.Mol. Gen. Genetics, Transgenic Expression of Two Marker Genes Under The Control of anArabiodopsis rbcSPromoter: Sequences Encoding the Rubisco Transit Peptide Increase Expression Levels, 218:78-86 (1989).
Schnieke, et al.Science, Human Factor IX Transgenic Sheep Produced by Transfer of Nuclei From Transfected Fetal Fibroblasts, 278:2130-2133 (1997).
McCabe et al.,BiolTechnology, Stable Transformation of Soybean (Glycine Max) by Particle Acceleration, 6: 923 (1988).
Christou, et al,Plant Physicol, Stable Transformation of Soybean Callus by DNA-Coated Gold Particles, 87:671-674 (1988).
McKently et al.,Plant Cell Rep, Agrobacterium-Mediated Transformation of Peanut (Arachis hypogaeaL.) Embryo Azes and the Development of Transgenic Plants, 14:699-703 (1995).
Grant, et al.Plant Cell Rep, Transformation of Peas (Pisum sativumL.) Using Immature Cotyledons, 15:254-258 (1995).
Bytebler, et al.,Proc Natl Acad Sci, T-DNA Organization in Tumor Cultures and Transgenic Plants of the Monocotyledon Asparagus Officinalis, (USA) 84:5354 (1987).
Wan & Lemaux,Plant Physiol, Gerneration of large Numbers of Independently Transformed Fertile Barley Plants, 10:37 (1994).
Rhodes, et al.,Science, Genetically Transformed Maize Plants from Protoplasts, 240:204 (1988).
Gordon-Kamm, et al.,Plant Cell, Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants, 2:603-618 (1990).
Fromm, et al.,BiolTechnology, Inheritance and Expression of Chimeric Genes in the Progeny of Transgenic Maize Plants, 8:833 (1990).
Koziel, et al.,BioTechnology, Field Performance of Elite Transgenic Maize Plants Expressing an Insecticidal Protein Derived fromBacillus thuringiensis, 11:194 (1993).
Armstrong, et al.Crop Science, Cell Biology & Molecular Genetics, 35:550-557 (1995).
Somers, et al.,BiolTechnology, Fertile, Transgenic Oat Plants, 10:15 89 (1992).
Horn, et al.,Plant Cell Rep, Transgenic Plants of Orchardgrass (Dactylis glomerataL.) From Protoplast, 7:469 (1988).
Park, et al.,Plant Mol. Biol., T-DNA Integration Into Genomic DNA of Rice Following Agrobacterium Inoculation of Isolated Shoot Apices, 32:1135-1148 (1996).
Abnedina, et al.,Aust. J. Plant Physiol., An Efficient Transformation System for the Australian Rice Cultivar, Jarrah, 24:133-141 (1997).

Affiliated with

Also associated with

Rating

Desaturase genes and uses thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Desaturase genes and uses thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Desaturase genes and uses thereof will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3618624